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Micropropagation of an endangered plant species: Coronopus navasii (Brassicaceae)

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Abstract

Plantlets of Coronopus navasii, an endangered species from SE Spain, were successfully regenerated from shoot and root segments excised from young seedlings. Initiation of multiple buds and development of leaves were obtained in MS modified medium plus l mg/l BAP and 0.1 mg/l NAA. Rooting was achieved by transfer of the isolated shoots to fresh MS medium without plant growth regulators. Plant survival of 47% was obtained six weeks after removal from in vitro culture conditions.

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Abbreviations

BAP:

6-benzylaminopurine

NAA:

naphthaleneacetic acid

MS medium:

see Murashige and Skoog 1962

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Authors and Affiliations

  1. Departamento Biología Vegetal, ETS Ingenieros Agrónomos, Universidad Politécnica, Ciudad Universitaria, E-28040, Madrid, Spain

    J. M. Iriondo & C. Pérez

Authors
  1. J. M. Iriondo
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  2. C. Pérez
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Communicated by I. Potrykus

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Iriondo, J.M., Pérez, C. Micropropagation of an endangered plant species: Coronopus navasii (Brassicaceae). Plant Cell Reports 8, 745–748 (1990). https://doi.org/10.1007/BF00272109

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  • DOI: https://doi.org/10.1007/BF00272109

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