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Somatic embryogenesis and plant regeneration from embryogenic callus of soybean, Glycine max L.

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Abstract

During induction of somatic embryogenesis from immature embryos of soybean, a smooth-shiny and a rough callus were obtained. The smooth-shiny type developed from callus derived from cotyledonary tissue and cultured on media containing 10 mg/l 2,4-D. The rough type was derived from immature embryos, cotyledons, hypocotyls and hypocotyl segments from germinated seedlings on a medium containing various growth regulators. Plants were obtained from the smooth-shiny type but rough callus did not differentiate into plants. The conditions for formation of both callus types and regeneration of mature soybean plants from the smooth-shiny type were defined.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indoleacetic acid

IBA:

indolebutyric acid

ABA:

abscicic acid

GA3:

gibberellic acid

BA:

benzyladenine

B5:

Gamborg et al.

LS:

Linsmaier & Skoog

MS:

Murashige & Skoog

P:

proline

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Authors and Affiliations

  1. Tissue Culture for Crops Project, Department of Botany, Colorado State University, 80523, Fort Collins, CO, USA

    T. D. Ghazi & M. W. Nabors

  2. Department of Botany, Punjab University, 160 014, Chandigarh, India

    H. V. Cheema

Authors
  1. T. D. Ghazi
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  2. H. V. Cheema
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  3. M. W. Nabors
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Communicated by F. Constabel

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Ghazi, T.D., Cheema, H.V. & Nabors, M.W. Somatic embryogenesis and plant regeneration from embryogenic callus of soybean, Glycine max L.. Plant Cell Reports 5, 452–456 (1986). https://doi.org/10.1007/BF00269640

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  • DOI: https://doi.org/10.1007/BF00269640

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