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Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. A tropical forage legume

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Abstract

Protoplasts were isolated from leaf mesophyll and cell suspensions of two accessions of Stylosanthes guianensis (Aubl.), a tropical forage legume. When cultured in VKM liquid culture medium, both types of protoplasts divided at a rate of 4–8%, and subsequently formed cell colonies. Protoplast-derived calluses produced numerous shoots when transferred to regeneration medium. Regenerated shoots could be easily rooted, and plantlets were transferred to soil. The effects of several factors on the efficiency of this protoplast system have been investigated.

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Abbreviations

BAP:

6-benzylaminopurine

GA3 :

Gibberellic acid

NAA:

Naphthalene acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

Zea:

Zeatin

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Authors and Affiliations

  1. Biotechnology Research Unit, CIAI-A.A. 6713, Colombia, CA, USA

    L. Szabados & W. M. Roca

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  1. L. Szabados
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  2. W. M. Roca
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Communicated by O. Gamborg

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Szabados, L., Roca, W.M. Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. A tropical forage legume. Plant Cell Reports 5, 174–177 (1986). https://doi.org/10.1007/BF00269111

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  • DOI: https://doi.org/10.1007/BF00269111

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