Skip to main content
SpringerLink
Log in

Cryopreservation of non-encapsulated embryogenic tissue of sweet potato (Ipomoea batatas)

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Embryogenic tissue of the sweet potato (Ipomoea batatas (L) LAM) genotype TIB 10 was established from in vitro axillary shoot tips on Murashige and Skoog (1962) medium supplemented with 5 μM 2,4-dichlorophenoxyacetic acid. Embryogenic aggregates of fresh mass 9.0–12 mg were subjected to a rapid freezing protocol in liquid nitrogen following sucrose preculture and varying degrees of dehydration. Up to 50% of embryogenic explants survived rapid freezing after preculture on 0.4 or 0.7M sucrose only. Dehydration with silica gel to moisture contents in the range 18–41% improved the survival after cryopreservation of embryogenic tissue. Tissue dehydrated for intermediate periods exhibited poor survival. Following freezing, embryogenic tissue appeared to develop normally, retaining its competence to produce mature embryos and plantlets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962) medium

References

  • Blakesley D, Al Mazrooei S, Henshaw GG (1995) Plant Cell Reports 15, 259–263

    Google Scholar 

  • Dereuddre J, Kaminski M (1992) J. Thermal Analysis 38: 1965–1978

    Google Scholar 

  • Dereuddre J, Blandin S, Hassen H (1991) Cryoletters 12: 125–134

    Google Scholar 

  • de Boucaud MT, Brison M, Negrier P (1994) Cryoletters 15: 151–160

    Google Scholar 

  • Dumet D, Engelmann F, Chabrillange N, Duval Y (1993) Plant Cell Reports 12: 352–355

    Google Scholar 

  • Grout BWW, Henshaw GG (1978) Ann. Bot. 42: 1227–1229

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of Bath, School of Biology and Biochemistry, BA2 7AY, Bath, UK

    D. Blakesley, S. Al Mazrooei, M. H. Bhatti & G. G. Henshaw

Authors
  1. D. Blakesley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Al Mazrooei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. H. Bhatti
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. G. Henshaw
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by M. R. Davey

Rights and permissions

Reprints and permissions

About this article

Cite this article

Blakesley, D., Al Mazrooei, S., Bhatti, M.H. et al. Cryopreservation of non-encapsulated embryogenic tissue of sweet potato (Ipomoea batatas). Plant Cell Reports 15, 873–876 (1996). https://doi.org/10.1007/BF00233160

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00233160

Keywords

Search

Navigation