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Isolation of an auxotrophic cell line of soybean (Glycine max) which requires asparagine or glutamine for growth

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Abstract

A suspension culture of haploid soybean cells was treated with ultra-violet light. From the mutagenized culture an auxotrophic cell line was isolated which grows on 1-B5 media supplemented with asparagine or glutamine. In the absence of these additives the cells cease to grow and die. Asparagine is unable to serve as a sole source of nitrogen, i.e., cannot substitute for nitrate and ammonia.

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References

  • Gamborg OL, Miller R A, Ojima K. (1968) Exp Cell Res 50: 151–158.

    Google Scholar 

  • Gebhardt C, Schnebli V, King, P J (1981) Planta 153: 81–89.

    Google Scholar 

  • King J, Horsch R B, Savage A D (1980) Planta 149: 480–484.

    Google Scholar 

  • Limberg M, Cress D, Lark K G (1979) Plant Physiol 63: 718–721.

    Google Scholar 

  • Müller A J, Grafe R (1978) Mol Gen Genet 161: 67–78.

    Google Scholar 

  • Strauss A, Bucher F, King P J (1981) Planta 153: 75–80.

    Google Scholar 

  • Weber G, Lark K G (1979) Theor Appl Genet 55: 81–86.

    Google Scholar 

  • Weber G, Lark K G (1980) Genetics 96: 213–222.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, The University of Utah, 84112, Salt Lake City, UT, USA

    E. Jill Roth & Karl G. Lark

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  1. E. Jill Roth
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  2. Karl G. Lark
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This work was supported by a grant from the National Institute of Environmental Health Services (01498)

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Roth, E.J., Lark, K.G. Isolation of an auxotrophic cell line of soybean (Glycine max) which requires asparagine or glutamine for growth. Plant Cell Reports 1, 157–160 (1982). https://doi.org/10.1007/BF00269187

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  • DOI: https://doi.org/10.1007/BF00269187

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