Summary
We have isolated Phe and Tyr tRNAs from Tetrahymena pyriformis mitochondria and have determined that these are “native” species, encoded by the mtDNA. A single gene for the tRNAPhe has been positioned 12–14 kbp from the left end of the linear Tetrahymena mtDNA, while duplicate tRNATyr genes have been localized within the inverted terminal repeats of this genome. Primary sequence analysis demonstrates that the tRNATyr has all of the characteristic primary and secondary structural features of a normal tRNA; however, the tRNAPhe displays several atypical features, including (i) replacement of the usual Tψ sequence by UC, (ii) a U - U pair in the TψC stem, and (iii) an extra 5′-nucleotide (U).
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Schnarel, M.N., Heinonen, T.Y.K., Young, P.G. et al. Phenylalanine and tyrosine transfer RNAs encoded by Tetrahymena pyriformis mitochondrial DNA: primary sequence, post-transcriptional modifications, and gene localization. Curr Genet 9, 389–393 (1985). https://doi.org/10.1007/BF00421610
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DOI: https://doi.org/10.1007/BF00421610