Summary
The cyanelle DNA from two different strains of Cyanophora paradoxa (strain LB555UTEX and strain 1555) was investigated.
The cyanelle DNA from both strains showed a buoyant density in neutral CsCI gradients of 1.692 g/cm3. The total molecular weight, as judged by restriction endonuclease analysis, of the two cyanelle DNAs differed. In strain LB555UTEX the size of the cyanelle DNA was equivalent to 127 ± 1 kb whereas in strain 1555 a size of 138 ± 1 kb was consistently found. The sizes of individual DNA fragments and the number of recognition sites for a particular restriction endonuclease appeared largely unrelated.
A high amount of cross hybridization, as judged by reciprocal heterologous DNA hybridizations, however indicated a high degree of sequence homology between the two cyanelle DNAs. Under comparable conditions, cyanelle DNA hybridized nearly exclusively with the dG+dC-rich rRNA transcription units from plastid DNAs. Up to now conserved restriction endonuclease recognition sites between the two cyanelle DNAs were only observed within the cyanelle rRNA genes which are present twice on both cyanelle DNAs.
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Abbreviations
- cyDNA:
-
cyanelle DNA
- rRNA:
-
ribosomal RNA
- rDNA:
-
ribosomal DNA
- kb:
-
kilobase pairs
- SSC:
-
0.15 M NaCl, 0.015 M sodium citrate, pH 7.0
- cpDNA:
-
chloroplast DNA
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Löffelhardt, W., Mucke, H., Crouse, E.J. et al. Comparison of the cyanelle DNA from two different strains of Cyanophora paradoxa . Current Genetics 7, 139–144 (1983). https://doi.org/10.1007/BF00365639
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DOI: https://doi.org/10.1007/BF00365639