Summary
We have sequenced the gene for cytochrome c oxidase subunit 1 (CO I) in Neurospora crassa mitochondrial DNA. The gene is coded by the same strand as the rRNA and tRNA genes. The coding sequence predicts a protein of 557 amino acids, starting with methionine, and ending with asparagine. Comparison to the N-terminal amino acid sequence of the mature protein (Werner et al. 1980) reveals that the methionine is located at position -2. No other upstream AUG codons have been found in frame. The C-terminal part of the gene is about 70 basepairs longer than the corresponding parts of the Saccharomyces and mammalian genes. The homology between the Neurospora coding sequence and those of yeast and mammals is very high. As compared to Saccharomyces, the introns il through i5 are absent.
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Abbreviations
- CO I, CO II, CO III:
-
subunit 1, 2, 3, resp. of cytochrome c oxidase
References
Agsteribbe E, Samallo J, Vries H de, Hensgens LAM, Grivell LA (1981) In: Kroon AM, Saccone C (eds) The organization and expression of the mitochondrial genome, pp 51–59
Anderson SA, Bankier AT, Barrell BG, Bruyn MHL de, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465
Barrell BG, Bankier AT, Drouin J (1979) Nature 282:189–194
Bertrand H, Werner S (1979) Eur Biochem 98:9–18
Birnboim HC, Doly J (1979) Nucl Acids Res 7:1513–1523
Bonitz SG, Coruzzi G, Thalenfeld BE, Tzagoloff A, Macino G (1980) J Biol Chem 255:11927–11941
Boogaart P van den, Dijk S van, Agsteribbe E (1982a) FEBS Lett 147:97–100
Boogaart P van den, Samallo J, Agsteribbe E (1982b) Nature 298:187–189
Browning KS, RajBhandary UL (1982) J Biol Chem 257:5253–5256
Capaldi RA, Vanderkooi G (1972) Proc Natl Acad Sci US 69:930–932
Coruzzi G, Tzagoloff A (1979) J Biol Chem 254:9324–9330
Goodman HM, MacDonald RJ (1980) In: Grossman L, Moldave K (eds) Methods in enzymology, vol. 65. Academic Press, pp 75–90
Grivell LS, Hensgens LAM, Osinga KA, Tabak HF, Boer PH, Crusius JBA, Laan JC van der, Haan M de, Horst G van der, Evers RF, Arnberg A (1982) In: Slonimski PP, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Press, pp 225–240
Heckman JE, Hecker LI, Schwartzbach SD, Barnett WE, Baumstark B, RajBhandary UL (1978) Cell 13:83–95
Heckman JE, Sarnoff J, Alzner-de Weerd B, Yin S, RajBhandary UL (1980) Proc Natl Acad Sci US 77:3159–3163
Hensgens LAM, Bonen L, Haan M de, Horst G van der, Grivell LA (1983) Cell (in press)
Hudspeth MES, Ainley WM, Shumard DS, Butow RA, Grossman LI (1982) Cell 30:617–626
Küntzel H, Heidrich M, Piechulla B (1981) Nucl Acids Res 9:1451–1461
Machleidt W, Werner S (1979) FEBS Lett 107:327–330
Macino G (1980) J Biol Chem 255:10563–10565
Maxam AM, Gilbert W (1980) In: Grossman L, Moldave K (eds) Methods in enzymology, vol 65. Academic Press, pp 499–560
Netter P, Jacq C, Carignani G, Slonimski PP (1982) Cell 28:733–738
Sebald W, Machleidt W, Otto J (1973) Eur J Biochem 38:311–324
Southern EM (1975) J Mol Biol 98:503–517
Terpstra P, Vries H de, Kroon AM (1977) In: Bandlow W, Schweyen RJ, Wolf K, Kaudewitz F (eds) Mitochondria 1977. De Gruyter, pp 291–302
Van't Sant P, Mak JFC, Kroon AM (1981) Eur J Biochem 121:21–26
Werner S, Machleidt W, Bertrand H, Wild G (1980) In: Kroon AM, Saccone C (eds) The organization and expression of the mitochondrial genome, pp 399–411
Yin S, Heckman JE, RajBhandary UL (1981) Cell 26:325–332
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de Jonge, J.C., de Vries, H. The structure of the gene for subunit I of cytochrome c oxidase in Neurospora crassa mitochondria. Current Genetics 7, 21–28 (1983). https://doi.org/10.1007/BF00365676
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DOI: https://doi.org/10.1007/BF00365676