Summary
Compared to wild type cells, strains bearing the pleiotropic regulatory mutations cgr4 or cas1 synthesize apocatalase T at a high rate when grown on high glucose. Like heme-deficient ole3 single mutants, ole3 cgr4 and ole3 cas1 double mutants accumulate no catalase T protein in vivo. This defect introduced by the ole3 mutation is cured by the addition of ALA. By use of the inhibitor actinomycin D we confirm previous findings that ole3 mutants lack catalase T mRNA and show that (i) the ole3 cgr4 and ole3 cas1 double mutants do accumulate catalase T mRNA or mRNA precursor, and (ii) the processing or translation of this RNA or the accumulation of apocatalase T depends on the presence of home.
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Abbreviations
- ALA:
-
δ-aminolevulinate
References
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Sledziewski, A., Rytka, J., Biliński, T. et al. Posttranscriptional heme control of catalase synthesis in the yeast Saccharomyces cerevisiae . Curr Genet 4, 19–23 (1981). https://doi.org/10.1007/BF00376781
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DOI: https://doi.org/10.1007/BF00376781