Abstract
The UFE1 gene of Saccharomyces cerevisiae was cloned, sequenced and characterized. The coding region of UFE1 is separated from the TMP1 gene on chromosome XV by 624 bp. Gene-disruption experiments demonstrated that UFE1 is essential for both the germination of ascospores and for vegetative growth. Translation of the UFE1 coding region generates a protein with significant similarity to cytokeratin and to the coiled-coil region of SED5, USO1 and restin, suggesting that it is involved in the secretory pathway and may also be related to intermediate filament-associated proteins.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 6 May/18 June 1996
Rights and permissions
About this article
Cite this article
Downing, T., Storms, R. Molecular analysis of UFE1, a Saccharomyces cerevisiae gene essential for spore formation and vegetative growth. Curr Genet 30, 396–403 (1996). https://doi.org/10.1007/s002940050148
Issue Date:
DOI: https://doi.org/10.1007/s002940050148