Summary
In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt + and mt − gametes contain the same amount of mitochondrial DNA and the two parental genomes persist for several days in the zygotes. The DNA of mt + origin is slowly eliminated during the period of zygote maturation. Light is required for total elimination of mt + mitochondrial DNA in the zygospores. Using appropriate restriction enzymes, we have been unable to detect methylation of the mitochondrial DNA during gametogenesis or zygospore formation. The possibility that the mt + mitochondria themselves are specifically eliminated in the course of zygote maturation is discussed.
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Alexander NJ, Gillham NW, Boynton JE (1974) Molec Gen Genet 130:275–290
Boynton JE, Harris EH, Burkhart BD, Lamerson PM, Gillham NW (1987) Proc Natl Acad Sci USA 84:2391–2395
Brand N, Arnold CG (1987) Endocyt C Res 4:275–288
Cavalier-Smith T (1976) Protoplasma 87:297–315
Colleaux L, Michel-Wolwertz M-R, Matagne RF, Dujon B (1990) Mol Gen Genet 223:288–296
Ehrlich M, Wang RYH (1981) Science 212:1350–1357
Feng TY, Chiang KS (1984) Proc Natl Acad Sci USA 81:3438–3442
Gillham NW, Boynton JE, Harris EH (1987) Curr Genet 12:41–47
Gorman DS, Levine RP (1965) Proc Natl Acad Sci USA 54:1665–1669
Kuroiwa T (1985) Microbiol Sci 2:267–270
Kuroiwa T, Kawano S, Nishibayashi S, Sato C (1982) Nature 298:481–483
Matagne RF, Rongvaux D, Loppes R (1988) Mol Gen Genet 214:257–262
Matagne RF, Michel-Wolwertz M-R, Munaut C, Duyckaerts C, Sluse F (1989) J Cell Biol 108:1221–1226
Meland S, Johansen S, Johansen T, Hangli K, Hangli F (1991) Curr Genet 19:55–60
Munaut C, Dombrowicz D, Matagne RF (1990) Curr Genet 18:259–263
Remacle C, Bovie C, Michel-Wolwertz M-R, Loppes R, Matagne RF (1990) Mol Gen Genet 223:180–184
Royer HD, Sager R (1979) Proc Natl Acad Sci USA 76:5794–5798
Sager R, Grabowy C, Sano H (1981) Cell 24:41–47
Surzycki S (1971) Methods Enzymol 23:67–73
VanWinkle-Swift KP (1977) J Phycol. 13:225–231
Vaughn KC, De Bonte LR, Wilson KG (1980) Science 208:196–198
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Communicated by K. P. VanWinkle-Swift
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Beckers, M.C., Munaut, C., Minet, A. et al. The fate of mitochondrial DNAs of mt + and mt − origin in gametes and zygotes of Chlamydomonas . Curr Genet 20, 239–243 (1991). https://doi.org/10.1007/BF00326238
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DOI: https://doi.org/10.1007/BF00326238