Summary
Mitochondrial (mt) DNAs from normal (N) and male sterile (S) cytoplasms of sugar been have been isolated and investigated by electron microscopy. The results showed that mtDNA was composed of a heterogeneous population of circular molecules. Their contour lengths varied from 0.28 to 51 μm, but unlike in the case of maize, a large difference was not observed in the distribution of molecular classes greater than 1.0 μm between N and S cytoplasms of sugar beet. On the other hand, N and S cytoplasms were shown to contain their own characteristic combinations of small circular mtDNA species with lengths between 0.28 μm and 0.6 μm. Mitochondrial DNAs from various sources of male-sterile cytoplasms were analyzed by agarose gel electrophoresis to determine the extent of cytoplasmic variation. Additional low molecular weight DNA bands appeared in all male-sterile lines examined, and as a result, three distinctive banding patterns were recognized. These data are in general agreement with those based upon restriction endonuclease digestion of mt and chloroplast DNAs and the genetic analysis of fertility restoration in test crosses.
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References
Boutry M, Faber AM, Charbonnier M, Briquet M (1984) Plant Mol Biol 3:445–452
Chétrit P, Mathieu C, Muller JP, Vedel F (1984) Curr Genet 8:413–421
Davis RW, Simon MN, Davidson N (1971) Methods Enzymol 21:413–439
Goblet JP, Boutry M, Duc G, Briquet M (1983) Plant Mol Biol 2:305–309
Goblet JP, Flamand MC, Briquet M (1985) Curr Genet 9:423–426
Handa H, Tsunewaki K, Kunisada T, Yamagishi H (1984) Mol Gen Genet 194:368–372
Hansen BM, Marcker KA (1984) Nucleic Acids Res 12:4747–4756
Herrmann RG, Bohnert HJ, Kowallik KV, Schmitt JM (1975) Biochim Biophys Acta 378:305–317
Kemble RJ, Bedbrook JR (1980) Nature (London) 284:565–566
Kinoshita T, Mikami T (1985) Proc Sugar Beet Res Asso Japan 27 (in press)
Leaver CJ, Gray MW (1982) Annu Rev Plant Physiol 33:373–402
Levings CS III, Shah DM, Hu WWL, Pring DR, Timothy DH (1979) In: Cummings D, Borst P, David I, Weissman S, Fox CF (eds) Extrachromosomal DNA. Academic Press, New York, pp 63–73 (ICN-UCLA Symposia on Molecular and Cellular Biology)
Lonsdale DM, Hodge TP, Fauron CMR (1984) Nucleic Acids Res 12:9249–9261
Mikami T, Sugiura M, Kinoshita T (1984 a) Curr Genet 8:319–322
Mikami T, Shinozaki K, Sugiura M, Kinoshita T (1984 b) Jpn J Genet 59:497–504
Mikami T, Kishima Y, Sugiura M, Kinoshita T (1985) Theor Appl Genet 71:166–171
Palmer JD, Shields CR (1984) Nature (London) 307:437–440
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Nature (London) 301:725–728
Powling A (1981) Mol Gen Genet 183:82–84
Powling A (1982) Heredity 49:117–120
Powling A, Ellis THN (1983) Theor Appl Genet 65:323–328
Pring DR, Levings CS III, Hu WWL, Timothy DH (1977) Proc Natl Acad Sci USA 74:2904–2908
Pring DR, Conde MF, Schertz KF, Levings CS III (1982) Mol Gen Genet 186:180–184
Sederoff RR (1984) Adv Genet 22:1–108
Sugiura M, Kusuda J (1979) Mol Gen Genet 172:137–141
Suzuki A, Oldemeyer RK, Erichsen AW (1974) In: Am Soc Sugar Beet Tech 18th General Meeting
Synenki RM, Levings CS III, Shah DM (1978) Plant Physiol 61:460–464
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Mikami, T., Harada, T. & Kinoshita, T. Heterogeneity of circular mitochondrial DNA molecules from sugar beet with normal and male sterile cytoplasms. Curr Genet 10, 695–700 (1986). https://doi.org/10.1007/BF00410918
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DOI: https://doi.org/10.1007/BF00410918