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Simultaneous measurements of DNA and protein content of microorganisms by flow cytometry

  • Biotechnology and Bioengineering
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European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

The simultaneous measurement of protein and DNA content of yeast on a cell by cell basis is described. A problem associated with partially overlapping fluorescence emission bands of the two fluorochromes is discussed. The rapid flow cytometric assay will be useful to monitor cell growth in industrial fermentation processes.

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Abbreviations

DNA:

Desoxyribonucleic acid

YEP:

yeast extract peptone

FITC:

fluorescein-iso-thiocyanate

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Authors and Affiliations

  1. Fraunhofer-Gesellschaft, Institut für Aerobiologie, D-5948, Schmallenberg, Federal Republic of Germany

    K.-J. Hutter

  2. Biophysics Systems GmbH, Im Eichenböhl 24, D-6140, Bensheim 3, Federal Republic of Germany

    H. E. Eipel

Authors
  1. K.-J. Hutter
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  2. H. E. Eipel
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Hutter, KJ., Eipel, H.E. Simultaneous measurements of DNA and protein content of microorganisms by flow cytometry. European J. Appl. Microbiol. Biotechnol. 6, 223–231 (1979). https://doi.org/10.1007/BF00508094

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  • DOI: https://doi.org/10.1007/BF00508094

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