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Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24

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Abstract

Rhodococcus strain I24 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We have identified a cosmid clone from I24 genomic DNA that is able to confer the ability to convert indene to indandiol upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalene-type dioxygenase. DNA sequence analysis of a portion of this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase. These genes will be useful for manipulating indene bioconversion in Rhodococcus strain I24.

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Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Bldg. 68-370, 77 Massachusetts Avenue, Cambridge, MA 02139, USA e-mail: asinskey@mit.edu Tel.: +1-617-253-6721 Fax: +1-617-253-8550, , , , , , US

    S. L. Treadway, E. Lankenau, P. A. Lessard & A. J. Sinskey

  2. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, , , , , , US

    K. S. Yanagimachi & G. Stephanopoulos

Authors
  1. S. L. Treadway
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  2. K. S. Yanagimachi
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  3. E. Lankenau
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  4. P. A. Lessard
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  5. G. Stephanopoulos
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  6. A. J. Sinskey
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Additional information

Received: 8 December 1998 / Received revision: 26 January 1999 / Accepted: 5 February 1999

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Treadway, S., Yanagimachi, K., Lankenau, E. et al. Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24. Appl Microbiol Biotechnol 51, 786–793 (1999). https://doi.org/10.1007/s002530051463

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  • DOI: https://doi.org/10.1007/s002530051463

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