Abstract
Gram-positive Rhodococcus erythropolis strain S1 formed enzymes for the degradation of phthalate when grown in a phthalate-containing minimal medium. The membrane fraction prepared from phthalate-grown cells by ultrasonication converted phthalate to protocatechuate as the final product. Using two membrane-bound enzymes, phthalate 3,4-dioxygenase (PO) and 3,4-dihydro-3,4-dihydroxyphthalate 3,4-dehydrogenase (PH), prepared by solubilization of the membrane fraction, 3,4-dihydroxyphthalate was selectively obtained from phthalata. Fe2+ and Mn2+ stimulated the formation of 3,4-dihydroxyphthalate by the membrane-bound PO and PH system.
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Suemori, A., Nakajima, K., Kurane, R. et al. Production of 3,4-dihydroxyphthalate from phthalate by a membrane-bound two-enzyme system from Rhodococcus erythropolis . Appl Microbiol Biotechnol 43, 470–472 (1995). https://doi.org/10.1007/BF00218451
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DOI: https://doi.org/10.1007/BF00218451