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Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111

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Summary

Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A.

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Authors and Affiliations

  1. Institut für Biologie I, Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle 1, W-7400, Tübingen, Federal Republic of Germany

    M. Greiner & G. Winkelmann

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  1. M. Greiner
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  2. G. Winkelmann
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Greiner, M., Winkelmann, G. Fermentation and isolation of herbicolin A, a peptide antibiotic produced byErwinia herbicola strain A 111. Appl Microbiol Biotechnol 34, 565–569 (1991). https://doi.org/10.1007/BF00167899

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  • DOI: https://doi.org/10.1007/BF00167899

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