Summary
Streptomyces coelicolor “Müller” DSM3030 excretes a lysozyme comprising both β-1,4-N-acetyl-and β-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S. coelicolor HP1, which exhibits about a twofold increase in lysozome production. The lysozyme-encoding genes (cel) from both strains were detected by oligodeoxynucleotide hybridization. The nucleotide sequence of the cel genes isolated from both strains was shown to be identical. The different levels of lysozyme production could not be correlated with any mutations at the cel gene locus. The cel gene isolated from the wild-type strain could not be expressed in some other species of Streptomyces. However, self-cloning of the cel gene into S. coelicolor DSM3030 and HP1 resulted in a 2.5-fold increase in lysozyme production.
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References
Arnold W, Pühler A (1988) A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing. Gene 70:171–179
Bayer E, Gugel KH, Hagele K, Hagenmaier H, Jessipow S, König WA, Zähner H (1972) Stoffwechselprodukte von Mikroorganismen. Phosphinothricin and Phosphinotricyl-alanyl-alanin. Helv Chim Acta 55:224–239
Bibb MJ, Findlay PR, Johnson MW (1984) The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences. Gene 30:157–166
Bibb MJ, Bibb MJ, Ward JM, Cohen SN (1985) Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous of Streptomyces. Mol Gen Genet 199:26–39
Birr E, Wohlleben W, Aufderheide K, Schneider T, Pühler A, Bräu B, Marquardt R, Wöhner G, Präve P, Schlingmann M (1989) Isolation and complementation of mutants of Streptomyces coelicolor “Müller” DSM3030 deficient in lysozyme production. Appl Microbiol Biotechnol 30:358–363
Chen EJ, Seeburg PH (1985) Supercoil sequencing: a fast simple method for sequencing plasmid DNA. DNA 4:165–170
Distler J, Braun C, Ebert A, Piepersberg W (1987) Gene cluster for streptomycin biosynthesis in Streptomyces griseus: analysis of a central including the major resistance gene. Mol Gen Genet 208:204–210
Ettlinger L, Corbatz R, Hütter R (1958) Zur Systematik der Actinomyceten. 4. Eine Arteinteilung der Gattung Streptomyces Waksman et Henrici. Microbiology 31:326–358
Felch JW, Inagami T, Hash JH (1975) The N,O-diacetylmuramidase of Chalaropsis species. V. The complete amino acid sequence. J Biol Chem 250:3713–3720
Fouche PB, Hash JH (1978) The N,O-diacetylmuramidase of Chalaropsis species. Identification of aspartyl and glutamyl residues in the active site. J Biol Chem 253:6787–6793
Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
Hohn B, Wu R (1979) In vitro packaging of lambda and cosmid DNA. Methods Enzymol 68:299–309
Hopwood DA, Kieser T, Wright HM, Bibb MJ (1983) Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol 129:2257–2269
Hughey VL, Johnson EA (1987) Antimicrobial activity of lysozyme against bacteria involved in food spoilage and foodborne disease. Appl Environ Microbiol 53:2165–2170
Ikemura H, Takagi H, Inoye M (1987) Requirement of prosequence for the production of active subtilisin E in Escherichia coli. J Biol Chem 262:7859–7864
Jaurin B, Cohen SN (1985) Streptomyces contain Escherichia coli-type A+T-rich promoters having novel structural features. Gene 39:191–201
Jollès P, Jollès J (1984) What's new in lysozyme research? Mol Cell Biochem 63:165–189
Kawata S, Takemura T, Yokogawa K (1983) Characterization of two N-acetylmuramidases from Streptomyces globisporus 18829. Agric Biol Chem 47:1501–1508
Kieser T, Hopwood BA, Wright HM, Thomas CJ (1982) pIJ101: a multicopy broad host range Streptomyces plasmid; functional analysis and development of DNA cloning vectors Mol Gen Genet 185:223–238
Leu WM, Wu SY, Lin JJ, Lo SJ, Lee Y-HW (1989) Analysis of the promoter region of the melanin locus from Streptomyces antibioticus. Gene 84:267–277
Lichenstein HS, Hastings AE, Langley KE, Mendiaz EA, Rohde MF, Elmore R, Zukowski MM (1990) Cloning and nucleotide sequence of the N-acetylmuramidase M1-encoding gene from Streptomyces globisporus. Gene 88:81–86
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbour, N. Y.
Morita T, Hara S, Matsushima Y (1978) Purification and characterization of lysozyme produced by Streptomyces erythraeus. J Biochem 83:893–903
Nakai R, Horinouchi S, Beppu T (1988) Cloning and nculeotide sequence of a cellulase gene, cas A, from an alkalophilic Streptomyces strain. Gene 65:229–238
Okanishi M, Suzuki K, Umezawa H (1974) Formation and reversion of streptomycete protoplasts: cultural conditions and morphological study. J Gen Microbiol 80:389–400
Robbins PW, Trimble RB, Wirth DF, Hering C, Maley F, Maley GF, Das R, Gibson BW, Royal N, Biemann K (1984) Primary structure of the Streptomyces enzyme endo-β-N-acetyl-glucosaminidase H. J. Biol Chem 259:7577–7583
Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Ann Rev Genet 13:319–353
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shiffman D, Mevarech M, Jensen SE, Cohen G, Aharonowitz Y (1988) Cloning and comparative sequence analysis of the gene coding for isopenicillin N synthase in Streptomyces. Mol Gen Genet 214:562–569
Shine J, Dalgarno J (1974) The 3′-terminal sequence of E. coli 16S ribosomal RNA: complementavity to nonsense triplets and ribosomal binding sites. Proc Natl Acad Sci USA 71:1342–1346
Silen JL, Frank D, Fujishige A, Bone R, Agard DA (1989) Analysis of pre-pro-alpha-lytic protease expression in Escherichia coli reveals that the pro region is required for activity. J Bacteriol 171:1320–1325
Strauch E, Wohlleben W, Pühler A (1987) Development of a plasmid-cloning system for Streptomyces viridochromogenes Tü494. J Basic Microbiol 27:449–455
Thompson CJ, Ward JM, Hopwood DA (1982) Cloning of antibiotic resistance and nutritional genes in streptomycetes. J Bacteriol 151:668–677
Thompson CJ, Movva N. Roa, Tizard R, Crameri R, Davies JE, Lauwereys M, Botterman J (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J 6:2519–2523
Vögtli M, Hütter R (1987) Characterisation of the hydroxystreptomycin phosphotransferase gene (sph) of Streptomyces glaucescens: nucleotide sequence and promoter analysis. Mol Gen Genet 208:195–203
Ward JM, Janssen GR, Kieser T, Bibb MJ, Buttner J, Bibb MJ (1986) Construction and characterization of a series of multicopy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Mol Gen Genet 203:468–478
Wohlleben W, Arnold W, Broer I, Hillemann D, Strauch E, Pühler A (1988) Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tü494 and its expression in Nicotiana tabacum. Gene 70:25–37
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Bräu, B., Hilgenfeld, R., Schlingmann, M. et al. Increased yield of a lysozyme after self-cloning of the gene in Streptomyces coelicolor “Müller”. Appl Microbiol Biotechnol 34, 481–487 (1991). https://doi.org/10.1007/BF00180575
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DOI: https://doi.org/10.1007/BF00180575