Summary
In a butanol batch fermentation the substrate consumption was increased threefold using in-situ product recovery by gas-stripping, in comparison with a control fermentation without product recovery. In a continuous fermentation in-situ recovery led to an increase in the biomass concentration, resulting in a threefold increase in productivity. The substrate consumption was increased by 10%. An external stripper was used as apparatus for the butanol recovery.
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Afschar AS, Biebl H, Schaller K, Schügerl K (1985) Production of acetone and butanol by Clostridium acetobutylicum in continuous culture with cell recycle. Appl Microbiol Biotechnol 22:394–398
Cysewski GR, Wilke CR (1977) Rapid ethanol fermentations using vacuum and cell recycle. Biotechnol Bioeng 19:1125–1143
Dale MC, Okos MR, Wankat PC (1985) Immobilized cell reactor with simultaneous product separation. II. Experimental reactor performance. Biotechnol Bioeng 27:943–952
Ennis BM, Marshall CT, Maddox IS, Paterson AHJ (1986) Continuous product recovery by in-situ gas stripping/condensation during solvent production from whey permeate using Clostridium acetobutylicum. Biotechnol Lett 8:725–730
Qureshi N, Maddox IS (1987) Continuous solvent production from whey permeate using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Enzyme Microb Technol 9:668–671
Schoutens GH, Nieuwenhuizen MCH, Kossen NWF (1985) Continuous butanol production from whey permeate with immobilized Clostridium beyerinckii LMD 27.6. Appl Microbiol Biotechnol 21:282–286
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Groot, W.J., van der Lans, R.G.J.M. & Luyben, K.C.A.M. Batch and continuous butanol fermentations with free cells: integration with product recovery by gas-stripping. Appl Microbiol Biotechnol 32, 305–308 (1989). https://doi.org/10.1007/BF00184979
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DOI: https://doi.org/10.1007/BF00184979