Summary
Poly(ethylene glycol) precipitation and two aqueous phase separation were examined as possible techniques for the large scale isolation of a microsomal fraction enriched in 11-α progesterone hydroxylase activity from the filamentous fungus Rhizopus nigricans. Both techniques concentrated the enzyme and increased its specific activity between 1.5 and 2.0-fold, but with low yield. Cell debris needed to be removed before PEG precipitation and also before two aqueous phase separation.
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Bonnerjea, J., Pontin, S., Hoare, M. et al. Poly(ethylene glycol) precipitation and two aqueous-phase separation of progesterone 11-α hydroxylase from Rhizopus nigricans . Appl Microbiol Biotechnol 27, 362–365 (1988). https://doi.org/10.1007/BF00251769
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DOI: https://doi.org/10.1007/BF00251769