Summary
The effects of oxygen tension and dilution rate on expression and stability of killer toxin chimeric plasmid (pADH-10A) in transformants of wine yeast (Montrachet 522) were investigated using a glucose-limited chemostat culture. Killer toxin activity increased as a function of dilution rate when transformants were grown in N2 or air, but not in 100% O2. Killer transformants grown in the presence of N2 had 2–4 fold and 10–15 fold higher toxin expression than that in the presence of air and 100% O2, respectively. Plasmid stability increased as a function of dilution rate, regardless of the oxygen tension used. However, transformants grown in air gave the highest plasmid stability.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aiba S, Koizumi J (1984) Effects of temperature on plasmid stability and penicillinase productivity in a transformant of Bacillus stearothermophilus. Biotech Bioeng 26:1026–1031
Bringer S, Finn RK, Sahm H (1984) Effect of oxygen on the metabolism of Zymomonas mobilis. Arch Microbiol 139:376–381
Bussey H, Meaden P (1985) Selection and stability of yeast transformants expressing cDNA of an M1 killer toxin-immunity gene. Curr Genetics 9:285–291
Carter IS, Dawes EA (1979) Effects of oxygen concentration and growth rate on glucose metabolism, poly-beta-hydroxybutyrate biosynthesis and respiration of Azotobacter beijerinckii. J Gen Microbiol 110:393–400
Dunn GM, Herbert RA, Brown CM (1979) Influence of oxygen tension on nitrate reduction by a Klebsiella sp. growing in chemostat culture. J Gen Microbiol 112:379–383
Hijortso MA, Bailey JE (1984) Plasmid stability in budding yeast populations: steady-state growth with selection pressure. Biotech Bioerg 26:528–536
Johson AW (ed) (1980) Oxygen and life. Whitstable Litho Ltd., Whitstable, Kent, UK
Lee FJ, Hassan HM (1987) Stability and expression of a plasmid containing killer toxin cDNA in batch and chemostat cultures of Saccharomyces cerevisiae. Biotech Bioeng (in press)
Ollis DF (1982) Industrial fermentations with (unstable) recombinant cultures. Philos Trans R Soc (London) B297:617–629
Author information
Authors and Affiliations
Additional information
Paper Number 10918 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC 27695. The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named. nor criticism of similar ones not mentioned.
Rights and permissions
About this article
Cite this article
Lee, FJ.S., Hassan, H.M. Effect of oxygen tension on stability and expression of a killer toxin chimeric plasmid in a chemostat culture of Saccharomyces cerevisiae . Appl Microbiol Biotechnol 27, 72–74 (1987). https://doi.org/10.1007/BF00257256
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00257256