Summary
The 11β- and 19-hydroxylation enzyme(s) of Pellicularia filamentosa IFO 6298 have been shown to be inducible by Reichstein's Substance S. By using the protein synthesis inhibitor, cycloheximide, in fermenter culture the effects of dissolved oxygen tension (DOT) on enzyme induction and enzyme expression have been separately investigated. For both hydroxylations, an optimum DOT for induction has been shown at 15% of saturation, while the optimum for expression is at 30% of saturation. The results have been verified in the absence of cycloheximide. Thus, maximum rates of hydroxylation are achieved when induction is performed at low DOT, followed by elevation to ensure maximum expression.
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Clark, T.A., Chong, R. & Maddox, I.S. The effect of dissolved oxygen tension on 11β- and 19-hydroxylation of Reichstein's Substance S by Pellicularia filamentosa . European J. Appl. Microbiol. Biotechnol. 14, 131–135 (1982). https://doi.org/10.1007/BF00497887
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DOI: https://doi.org/10.1007/BF00497887