Summary
Fermentation of the fused protein staphylococcal protein A-human insulin-like growth factor I (SpA-IGF-I) was investigated. The fused protein was expressed in Staphylococcus aureus and secreted to the culture medium. The production of SpA-IGF-I was growth related and started after a short lag phase. Examination of the product quality by SDS-PAGE after IgG affinity purification showed that the product was partially degraded. Exoproteases appeared later than SpA-IGF-I and were responsible for some, but probably not all, of the degradation of the product. It was possible to influence the exoprotease activity and thereby the product degradation by varying the cultivation conditions.
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Hellebust, H., Uhlén, M. & Enfors, SO. Role of proteases in fermentation of the fused protein staphylococcal protein A-human insulin-like growth factor I in Staphylococcus aureus . Appl Microbiol Biotechnol 28, 258–262 (1988). https://doi.org/10.1007/BF00250451
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DOI: https://doi.org/10.1007/BF00250451