Summary
The effect of ammonium ions in the medium on production of thiostrepton byStreptomyces laurentii was investigated. In batch cultures, the excessive initial concentration of ammonium ions inhibited thiostrepton production. Moderate feeding of ammonia accelerated, however, not only microbial growth but also thiostrepton production. Fed-batch cultures with various feed rates of ammonia and a kinetic study clarified the effect of ammonium ion consumption rate on thiostrepton production. A modified kinetic model is proposed that takes product inhibition and the influence of maximum thiostrepton content into account.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Donovick R, Vandeputte J (1961) US patent no. 2982689
Suzuki T, Yasuda T, Yamane T, Shimizu S (1986a) Detection and automatic control of ammonium ion concentration in microbial culture with an ammonium ion selective electrode. J Ferment Technol 64:63–70
Suzuki T, Yamane T, Shimizu S (1986b) Mass production of poly-β-hydroxybutyric acid by fully automatic fed-batch culture of methylotroph. Appl Microbiol Biotechnol 23:322–329
Suzuki T, Yamane T, Shimizu S (1986c) Kinetics and effect of nitrogen source feeding on production of poly-β-hydroxybutyric acid by fed-batch culture. Appl Microbiol Biotechnol 24:366–369
Suzuki T, Yamane T, Shimizu S (1987) Mass production of thiostrepton by fed-batch culture ofStreptomyces laurentii with pH-stat modal feeding of multi-substrate. Appl Microbiol Biotechnol 25:526–531
Trejo WH, Pluscec J, Brown WE, Meyers E (1985) Japanese patent no. 60-40837
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Suzuki, T., Yamane, T. & Shimizu, S. Effect of ammonium feeding on production of thiostrepton by fed-batch culture. Appl Microbiol Biotechnol 28, 188–192 (1988). https://doi.org/10.1007/BF00694310
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00694310