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The immune response of inbred mouse strains to DNP-BGG

I. The effect of dose and adjuvant

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Abstract

The immune response of six inbred mouse strains (SJL, A, C57BL/6, CBA, BALB/c, and DBA/1) to DNP56BGG was tested under three separate immunization schedules: 1 Μg DNP-BGG in 1 mg Al(OH)3 adjuvant, 50 Μg DNP-BGG in 1 mg A1(OH)3 adjuvant, and 1 Μg DNP-BGG in complete Freund's adjuvant. Individual serum samples were titered using a modified Farr assay. It was found that the first schedule allowed classification of the mice into responder (SJL, A) and nonresponder (C57BL/6, CBA, BALB/ c, DBA/1) strains. The second schedule produced quantitative as well as qualitative differences among the strains and allowed classification of the mice into higher-responder (SJL, A), intermediate-responder (C57BL/6, CBA, BALB/c), and low-responder (DBA/1) categories. When complete Freund's adjuvant was used in the third schedule, the differences among strains became insignificant. The sera from each strain were pooled and assayed for relative antibody affinity and IgM content. Both of these parameters were dependent largely on the dose of antigen and type of adjuvant used, rather than on the particular mouse strain being studied. The mechanism of adjuvant action, and possible cell interactions in the genetic control of the immune response, are discussed.

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Abbreviations

CFA:

complete Freund's adjuvant

PBS:

sodium phosphate-buffered saline, pH 7.0

BGG:

bovine gamma globulin (Cohn fraction II)

DNP-BGG:

2,4-dinitrophenyl-bovine gamma globulin

BOC:

butyloxycarbonyl group

References

  • Benacerraf, B. and Katz, D.H.: The histocompatibility-linked immune response genes.Adv. Cancer Res. 25:121–173, 1975

    Google Scholar 

  • Benacerraf, B. and McDevitt, H.O.: Histocompatibility linked immune genes.Science 175:273–279, 1972

    Google Scholar 

  • Del Guercio, P., Thobie, N., Poirier, M.F.: Failure of Farr test in detecting IgM response to dinitrophenyl in mice.J. Immunol. Methods 11:49–53, 1976

    Google Scholar 

  • Desaymard, C. and Feldmann, M.: Lack of requirement for cell cooperation in the antibody response to DNP conjugated to levan.Cell. Immunol. 16:106–114, 1975

    Google Scholar 

  • Dresser, D.W.: The role of T cells and adjuvant in the immune response of mice to foreign erythrocytes.Eur. J. Immunol. 2:50–57, 1972

    Google Scholar 

  • Eisen, H.N., Garsten, M.E., and Belman, S.: Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the precipitin reaction.J. Immunol. 73:296–308, 1954

    Google Scholar 

  • Freed, J.H., Deak, B.D., and Bechtol, K.B.: Mapping of the genetic control of murine immune response to low doses of DNP conjugates of bovine γ-globulin and ovomucoid.Fed. Proc. 32:995a, 1973

    Google Scholar 

  • Gasser, D.L. and Silvers, W.K.: Genetic control of the immune response in mice. An association between H-2 type and reaction to H-Y.J. Immunol. 106:875–876, 1971

    Google Scholar 

  • Gershon, R.K. and Paul, W.E.: Effect of thymus-derived lymphocytes on amount and affinity of anti-hapten antibody.J. Immunol. 106:872–874, 1971

    Google Scholar 

  • Green, I.: Genetic control of immune responses.Immunogenetics 1:4–21, 1974

    Google Scholar 

  • Green, I., Benacerraf, B., and Stone, S.H.: The effect of the amount of mycobacterial adjuvants on the immune response of strain 2, strain 13 and Hartley strain guinea pigs to DNP-PLL and DNP-GL.J. Immunol. 103:403–412, 1969

    Google Scholar 

  • Hamoka, T. and Katz, D.H.: Cellular site of action of various adjuvants in antibody responses to hapten-carrier conjugates.J. Immunol. 111:1554–1563, 1973)

    Google Scholar 

  • Howard, J.G., Christie, G.H., and Scott, M.T.: Biological effects ofCorynebacterium parvum. IV. Adjuvant and inhibitory activities on B lymphocytes.Cell. Immunol. 7:290–301, 1973

    Google Scholar 

  • Ishibashi, T., Tanaka, A., Kohashi, O., and Sugiyama, K.: The mode of action of immunological adjuvants. II. Generation of a potent precursor of antibody-forming cells by adjuvant.Jap. J. Exp. Med. 45:147–154, 1975

    Google Scholar 

  • Karush, F.: Affinity and the immune process.Ann. N.Y. Acad. Sci. 169:56–71, 1970

    Google Scholar 

  • Katz, F.E. and Steward, M.W.: The genetic control of antibody affinity in mice.Immunology 29:543–548, 1975

    Google Scholar 

  • Katz, F.E. and Steward, M.W.: Studies on the genetic control of antibody affinity: the independent control of antibody levels and affinity in Biozzi mice.J. Immunol. 177:477–479, 1976

    Google Scholar 

  • Kim, Y.T., Greenbaum, D., Davis, P., Fink, S.A., Werblin, T.P., and Siskind, G.W.: Studies on the control of antibody synthesis. IX. Effect of boosting on antibody affinity.J. Immunol. 114:1302–1306, 1975

    Google Scholar 

  • Kim, Y.T. and Siskind, G.W.: Studies on the control of antibody synthesis. VI. Effect of antigen dose and time after immunization on antibody affinity and heterogeneity in the mouse.Clin. Exp. Immunol. 17:329–338, 1974

    Google Scholar 

  • Klein, J.:Biology of the Mouse Histocompatibility-2 Complex. pp. 424–428, Springer-Verlag, New York, 1975

    Google Scholar 

  • Levine, B.B. and Vaz, N.M.: Effect of combinations of inbred strain, antigen, and antigen dose on immune responsiveness and reagin production in the mouse.Int. Arch. Allergy Appl. Immunol. 39:156–171, 1970

    Google Scholar 

  • Little, J.R. and Eisen, H.N.: Preparation of immunogenic 2,4-dinitrophenol and 2,4,6-trinitrophenol proteins.In C.A. Williams and M.W. Chase (eds.):Methods in Immunology and Immunochemistry, Vol. I. pp. 128–133, Academic Press, New York, 1968

    Google Scholar 

  • Macario, A.J.L. and De Macario, E.C.: Sequential changes and persistence of antibody molecules during the immune response with special reference to the binding properties of the antigen-combining site.Immunochemistry 12:249–262, 1975

    Google Scholar 

  • McDevitt, H.O. and Chinitz, A.: Genetic control of the antibody response: relationship between immune response and histocompatibility (H-2) type.Science 163:1207–1208, 1969

    Google Scholar 

  • Munro, A.J. and Taussig, M.J.: Two genes in the major histocompatibility complex control immune response.Nature (Lond.) 256:103–106, 1975

    Google Scholar 

  • Paul, W.E. and Elfenbein, G.J.: An expression for the calculation of relative affinities of antibody-ligand interactions.J. Immunol. 114:216–264, 1975

    Google Scholar 

  • Schmitt-Verhulst, A.M., Mozes, E., and Sela, M.: Genetic control of the immune response to a thymus-independent synthetic polypeptide.Immunogenetics 1:357–369, 1974

    Google Scholar 

  • Scott, M.T.: Biological effects of the adjuvant Corynebacterium parvum. II. Evidence for macrophage-T-cell interaction.Cell Immunol. 5:469–479, 1972

    Google Scholar 

  • Sebaldt, R.J.: On the estimation of antibody titres by the haemagglutination technique.Immunochemistry 13:473, 1976

    Google Scholar 

  • Seppälä, I.J.T.: Disturbance of hapten-antibody equilibria by ammonium sulphate solutions. A source of error in antibody affinity determinations.J. Immunol. Methods 9:135–140, 1975

    Google Scholar 

  • Steward, M.W. and Petty, R.E.: Evidence for the genetic control of antibody affinity from breeding studies with inbred mouse strains producing high and low affinity antibody.Immunology 30:789–797, 1976

    Google Scholar 

  • Stupp, Y., Paul, W.E., and Benacerraf, B.: Structural control of immunogenicity. II. Antibody synthesis and cellular immunity in response to immunization with mono-ɛ-oligo-L-lysine.Immunology 21:583–494, 1971

    Google Scholar 

  • Tanaka, A., Ishibashi, T., Kohashi, O., Koga, T., and Sugiyama, K.: The mode of action of immunological adjuvants. I. A new method to assess the adjuvant effect of substances as represented by mycobacterial adjuvants.Jap. J. Exp. Med. 45:139–146, 1975

    Google Scholar 

  • Taniguichi, M., Tada, T., and Tokuhisa, T.: Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. III. Dual gene control of the T-cell-mediated suppression of the antibody response.J. Exp. Med. 144:20–31, 1976

    Google Scholar 

  • Vaz, N.M. and Levine, B.B.: Immune responses of inbred mice to repeated low doses of antigen: relationship to histocompatibility (H-2) type.Science 168:852–854, 1970

    Google Scholar 

  • Vaz, N.M., Philips-Quagliata, J.M., Levine, B.B. and Vaz, E.: H-2 linked genetic control of immune responsiveness to ovalbumin and ovamucoid.J. Exp. Med. 134:1335–1348, 1971

    Google Scholar 

  • Zav'yalov, V.P., Troitsky, G.V., Demchenko, A.P. and Generalov, I.V.: Temperature and pH dependent changes of immunoglobulin G structure.Biochim. Biophys. Acta 386:155–167, 1975

    Google Scholar 

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  1. Department of Biochemistry and Biophysics, Iowa State University, 50011, Ames, Iowa

    Robert C. Newton & Carol M. Warner

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  1. Robert C. Newton
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  2. Carol M. Warner
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Newton, R.C., Warner, C.M. The immune response of inbred mouse strains to DNP-BGG. Immunogenetics 4, 449–462 (1977). https://doi.org/10.1007/BF01575680

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  • DOI: https://doi.org/10.1007/BF01575680

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