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HLA-DQ RFLP variants of five HLA-DQw2-bearing major histocompatability complex extended haplotypes

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Abstract

We have analyzed genomic DNA in a large number of independent examples of five HLA-DQw2-bearing extended haplotypes for their associated subtypes by restriction fragment length polymorphism (RFLP) using DRB, DQA, and DQB probes after Taq I and Pst I digestion and Southern blotting. In addition to three previously described HLA-DQw2 subtypes, DQw2a, DQw2b, and DQw2c, we observed a fourth subtype, HLA-DQw2d, characterized by 5.8 kilobase (kb) DRB/Taq I, 2.4, 2.3, and 1.8 kb DQB/Taq I, and 8.0 and 2.3 kb DQA/Pst I fragments. All 22 independent examples of the extended haplotype [HLA-B8,SCO1,DR3] carried DQw2a and all 11 independent examples of [HLA-B18,F1C30,DR3] carried DQw2b. In addition, all independent examples (21 and 4, respectively) of two DR7-carrying extended haplotypes, [HLA-B44,FC31,DR7] and [HLA-Bw47,FC91,0,DR7], carried DQw2c and all independent examples of [HLA-Bw57,SC61,DR7] carried DQw2d. Our results show that the DNA in the DR/DQ region of extended haplotypes is relatively fixed and that different DQw2 subtypes characterize different DQw2-bearing extended haplotypes.

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References

  • Alper, C. A., Awdeh, Z. L., Raum, D. D., and Yunis, E. J.: Extended major histocompatability complex haplotypes in man: role of alleles analogous to murine t mutants. Clin Immunol Immunopathol 24: 276–285, 1982

    Google Scholar 

  • Alper, C. A., Awdeh, Z. L., Raum, D. D., and Yunis, E. J.: Possible human analogs of the murine T/t complex. Exp Clin Immunogenet 2: 125–136, 1985

    Google Scholar 

  • Auffray, C., Korman, A. J., Roux-Dosseto, M., Bono, R., and Strominger, J. L.: A cDNA clone for the heavy chain of the human B cell alloantigen CD1: strong sequence homology to the HLA-DR heavy chain. Proc Natl Acad Sci USA 79: 6337–6341, 1982

    Google Scholar 

  • Bontrop, R. E., Carpenter, C. B., Walford, R., Sekiguchi, S., Bignon, J. D., and Cohen, D.: RFLP standardization report for DQ Alpha/Taq I. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatability Testing 1987, pp. 815–816, Springer, New York, 1989

    Google Scholar 

  • Carroll, M. C., Campbell, R. D., Bentley, D. R., and Porter, R. R.: A molecular map of the human major histocompatability complex class III region linking complement genes C4, C2 and factor B. Nature 307: 237–241, 1984

    Google Scholar 

  • Chambon-Thomsen, A., Calot, M., Sommer, E., Ohayon, E., Goeken, N., Kaplan, C., Mattiuz, P. L., Meniccuci, A., Cross, R., Tait, B., Buc, M., Jeannet, M., Irle, C., Mayer, S., Tongio, M. M., Contu, L., Purpura, M., Nikaein, A., Mervart, H., Sullivan, K., Schweizer, R., Hansen, J. A., Du Toit, E., and Hammond, M. G.: Antigen Society # 26 report (DR3, DR7, DQw2). Part 1. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatability Testing 1987, pp. 255–258, Springer, New York, 1989a

    Google Scholar 

  • Chambon-Thomsen, A., Calot, M., Goeken, N., Kaplan, C., Martiuz, P. L., and Ohayon, E.: Antigen Society # 26 report (DR3, DR7, DQw2). Part 3: serologic definition of HLA-DR7. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatability Testing 1987, pp. 260–263, Springer, New York, 1989b

    Google Scholar 

  • Cohen, D., Simons, M. J., Lalouel, J. M., and Dupont, B.: Nomenclature for HLA-RFLP. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatability Testing 1987, ppp. 67–71, Springer, New York, 1989

    Google Scholar 

  • Dasgupta, J. D., Relias, V., Awdeh, Z. L., Alper, C. A., and Yunis, E. J.: Two variants of DRw52, DR3 and DQw2 specificities distinguish two different DR3-bearing extended haplotypes. Hum Immunol 21: 133–142 1988

    Google Scholar 

  • Dausset, J.: The major histocompatability complex in man. Past, present and future concepts. Science 2: 1469–1474, 1981

    Google Scholar 

  • Davis, L. G., Dibner, M. D., and Battey, J. F.: Preparation of DNA from future eukaryotic cells: general method. In L. Davism, M. Deibner and J. Battey (eds.): Basic Methods in Moleculr Biology, pp. 44–46, Elsevier, New York, 1986

    Google Scholar 

  • Dawkins, R. L., Martin, E., Andreas-Zietz, A., Keller, E., Partanen, J., Arnaiz-Villena, A., Vicario, J. L., and Alper, C. A. Linkage disequilibrium, interlocus association and ancestral haplotypes. In B. Dupont (ed.): Immunobiology of HLA, Vol. I, Histocompatability Testing 1987, pp. 891–892, Springer, New York, 1989

    Google Scholar 

  • Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analyt Biochem 137: 226–267, 1984

    Google Scholar 

  • Hitman, G. A., Sachs, J., Cassel, P., Awad, J., Botazzo, G. F., Tarn,A. C., Schwartz, G., Monson, J. P., and Festenstein, H.: A DR3-related DXα gene polymorphism strongly associates with insulin-dependent diabetes mellitus. Immunogenetics 23: 47–51, 1986

    Google Scholar 

  • Hyldig-Nielsen, J. J., Trucco, M., Andreas-Zietz, A., Keller, E., Carcassi, C., Takada, A., and Svejgaard, A.: RFLP standardization report for DQ Alpha/ Pst I. In (ed.): Immunobiology of HLA, Vol I, Histocompatability Testing 1987, pp. 809–810, Springer, New York, 1989

    Google Scholar 

  • Kaplan, C., Chambon-Thomsen, A., Goeken, N., Mattiuz, P. L., and Meniccuci, A.: Antigen Society # 26 report (D3, DR7, DQw2). Part 2: HLA-DR3. In B. Dupont (ed.): Immunobiology of HLA VVol 1, Histocompatability Testing 1987, pp. 259–260, Springer, New York, 1989

    Google Scholar 

  • Keller, E., Andreas-Zietz, A., Albert, E. D., Coppin, H., Contu, L., Takada, A., and Trucco, M.: RFLP standardization report for DQB/PstI. In B. Dupont (ed.): Immunobiology of HLA Vol I, Histocompatability Testing 1987, pp. 615–816, Springer, New York, 1989

    Google Scholar 

  • Klein, J.: Evolution and function of the major histocompatibility system: facts and speculations. In D. Gotze (ed.): The Major Histocompatibility System in Man and Animals, pp. 339–378, Springer, Berlin, 1977

    Google Scholar 

  • Long, E. O., Wake, C. T., Strubin, M., Gross, N., Acolla, R. S., Carrel, S., and Mach, B.: Isolation of distinct cDNA clones encoding HLA-DRB chains by use of an expression assay. Proc Natl Acad Sci USA 79: 7465–7469, 1982

    Google Scholar 

  • Marcadet, A., Martell, M., Cohen, D., Bignon, J. D., Kobayashi, K., Carpenter, C. B., and Walford, R. L.: RFLP standardization report for DQ Beta/Taq I. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatability Testing 1987, pp. 620–622, Springer, New York, 1989

    Google Scholar 

  • Mattiuz, P. L., Chambon-Thomsen, A., Goeken, N., Kaplan, C., and Calot, M.: Antigen Society # 26 report (DR3, DR7, DQw2). Part 4: HLA-DQw2. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatability Testing 1987, pp. 263–266, Springer, New York, 1989

    Google Scholar 

  • Raum, D., Awdeh, Z., Yunis, E. J., Alper, C. A., and Gabbay, K. H.: Extended major histocompatability complex haplotypes in Type I diabetes mellitus. J Clin Invest 74: 449–454, 1984

    Google Scholar 

  • Serjeantson, S. W., Kohonen-Corish, M. R. J., Dunckey, H., and Reid, M. A.: HLA class II RFLPs are haplotype-specific. Cold Spring Harbor Symp Quant Biol 50: 83–89, 1986

    Google Scholar 

  • Spiro, R. C., DeMartino, J. L., Boto, W., Lazarus, H., and Humpreys, R. E.: Comparison of membrane proteins of Burkitt's lymphoma and EBV-transformed B-lymphoblastoid cell lines and of Con-A activated T lymphocytes and T lymphoblastoid cell lines. Leuk Res 3: 315–327, 1979

    Google Scholar 

  • Wescott, M. Z., Awdeh, Z. L., Yunis, E. J., and Alper, C. A.: Molecular analysis distinguishes two HLA-DR3-bearing major histocompatibility complex extended haplotypes. Immunogenetics 26: 370–374, 1987

    Google Scholar 

  • White, P. C., Grossberger, D., Onufer, B. J., Chaplin, D. D., New, M. I., Dupont, B., and Strominger, J. L.: Two genes encoding 21-hydroxylase are located near the genes encoding the fourth component of complement in man. Proc Natl Acad Sci USA 82: 1089–1093, 1985

    Google Scholar 

  • Winchester, R., Matthews, E., Amesland, F., Mazzilli, M. C., Fernandez, N., Kienzle, B., and Knowles, R. W.: DR7: 2-D gel patterns. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatibility Testing 1987, pp. 396–397, Springer, New York, 1989

    Google Scholar 

  • Yang, S. Y., Milford, E., Hämmerling, U., and Dupont, B.: Description of the reference panel of B-lymphoblastoid cell lines for factors of the HLA system: the B-cell line panel designed for the Tenth International Histocompatibility Workshop. In B. Dupont (ed.): Immunobiology of HLA, Vol I, Histocompatibility Testing 1987, pp. 1–19, Springer, New York, 1989

    Google Scholar 

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Authors and Affiliations

  1. Division of Immunogenetics, Dana-Farber Cancer Institute, Boston, Massachusetts, USA

    Juan J. Yunis, Scarlet J. Lechin & Edmond J. Yunis

  2. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA

    Juan J. Yunis, Scarlet J. Lechin, Zuheir Awdeh & Edmond J. Yunis

  3. American Red Cross Northeast Region, Dedham, Massachusetts, USA

    Mark Wescott, Sharon M. Alosco & Edmond J. Yunis

  4. The Center for Blood Research, Boston, Massachusetts, USA

    Zuheir Awdeh, Chester A. Alper & Edmond J. Yunis

  5. Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA

    Chester A. Alper

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  1. Juan J. Yunis
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  2. Mark Wescott
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  3. Scarlet J. Lechin
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  4. Sharon M. Alosco
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  5. Zuheir Awdeh
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  6. Chester A. Alper
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  7. Edmond J. Yunis
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Yunis, J.J., Wescott, M., Lechin, S.J. et al. HLA-DQ RFLP variants of five HLA-DQw2-bearing major histocompatability complex extended haplotypes. Immunogenetics 32, 88–95 (1990). https://doi.org/10.1007/BF00210445

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  • DOI: https://doi.org/10.1007/BF00210445

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