Abstract
Two interleukin-1 polypeptides, α and β, are known, and cDNAs corresponding to each have been described. Genomic cloning and Southern blotting experiments suggest that in the mouse each is encoded by a gene present in one copy per haploid genome. Analysis of a panel of somatic cell hybrids carrying various mouse chromosomes on a constant Chinese hamster background indicates that both genes map to mouse chromosome 2. Further, analysis of the inheritance of DNA restriction fragment length polymorphisms associated with each gene in recombinant inbred strains of mice shows the two loci to be tightly linked to one another, and to lie approximately 4.7 centimorgans distal to B2m (beta-2 microglobulin). We have named the locus encoding IL-1 α Il-1α and the locus encoding IL-1 β Il-1b.
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Auron, P. E., Webb, A. C., Rossenwasser, L. J., Mucci, S. E., Rich, A., Wolff, S. M., and Dinarello, C. A.: Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc. Natl. Acad. Sci. U.S.A. 81: 7907–7911, 1984
Beck, G., Habicht, G. S., Benach, J. L., and Miller, F.: Interleukin 1: A common endogenous mediator of inflammation and the local Schwartzman reaction. J. Immunol. 136: 3025–3031, 1986
Blatt, C., Harper, M. E., Franchini, G., Nesbitt, M. E., and Simon, M. I.: Chromosomal mapping of c-fes and c-src genes. Mol. Cell. Biol. 4: 978–981, 1984
Chaplin, D. D., Woods, D. E., Whitehead, A. S., Goldberger, G., Colten, H. R., and Seidman, J. G.: Molecular map of the murine S region. Proc. Natl. Acad. Sci. U.S.A. 80: 6947–6951, 1983
Clark, B. D., Collins, K. L., Gandy, M. S., Webb, A. C., and Auron, P. E.: Genomic sequence for human prointerleukin 1 beta: Possible evolution from a reverse transcribed prointerleukin 1 alpha. Nucleic Acids Res. 14: 7898–7914, 1986
D'Eustachio, P., Fein, B., Michaelson, J., and Taylor, B.: The α-globin pseudogene on mouse chromosome 17 is closely linked to H-2. J. Exp. Med. 159: 958–963, 1984
D'Eustachio, P., Kristensen, T., Weisel, R. A., Riblet, R., Taylor, B. A., and Tack, B. F.: Chromosomal location of the genes encoding complement components C5 and factor H in the mouse. J. Immunol. 137: 3990–3995, 1986
Durum, S. K., Schmidt, J. A., and Oppenheim, J. J.: Interleukin-1: an immunological perspective. Annu. Rev. Immunol. 3: 263–287, 1985
Epstein, R., Lehman, K., and Cohn, M.: Induction of λ1-immunoglobu-lin is determined by a regulatory gene (rλ1) linked (or identical) to the structural (cλ1) gene. J. Exp. Med. 157: 1681–1686, 1983
Fuhlbrigge, R. C., Chaplin, D. D., Kiely, J.-M., and Unanue, E. R.: Regulation of interleukin-1 gene expression by adherence and lipopolysaccharide. J. Immunol. 138: 3799–3802, 1987
Furutani, Y., Notake, M., Fukui, T., Ohue, M., Hideki, N., Yamada, M., and Nakamura, S.: Complete nucleotide sequence of the gene for human interleukin 1 alpha. Nucleic Acids Res. 14: 3167–3179, 1986
Goodfellow, P. N., Jones, E. A., van Heyningen, V., Solomon, E., Bobrow, M., Miggiano, V., and Bodmer, W. F.: The β 2-microglobulin gene is on chromosome 15 and not in the HLA-region. Nature 254: 267–269, 1975
Gray, P. W., Glaister, D., Chen, E., Goeddel, D., and Pennica, D.: Two interleukin 1 genes in the mouse: Cloning and expression of the cDNA for routine IL-1 β . J. Immunol. 137: 3644–3648, 1986
Grosveld, F. G., Lund, T., Murray, E. J., Mellor, A. L., Dahl, H. H., and Flavell, R. A.: The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Res. 10: 6715–6732, 1982
Ish-Horowicz, D. and Burke, J.: Rapid and efficient cosmid cloning. Nucleic Acids Res. 9: 2989–2998, 1981
Kampschmidt, R. F.: The numerous biological manifestations of interleukin-1. J. Leukocyte Biol. 36: 341–356, 1984
Lentner, C. (ed.): Geigy Scientific Tables, volume 2, pp. 89–102, Ciba-Geigy, West Caldwell, New Jersey, 1982
Lomedico, P. T., Gubler, U., Hellmann, C. P., Dukovich, M., Giri, J. G., Pan, Y.-C. E., Collier, K., Semionow, R., Chua, A. O., and Mizel, S. B.: Cloning and expression of routine interleukin-1 cDNA in Escherichia coli. Nature 312: 458–462, 1984
Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular Cloning: A Laboratory Manual, pp. 149–162, 382–386, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
March, C. J., Mosley, B., Larsen, A., Cerretti, D. P., Braedt, G., Price, V., Gillis, S., Henney, C. S., Kronheim, S. R., Grabstein, K., Conlon, P. J., Hopp, T. P., and Cosman, D.: Cloning, sequence, and expression of two distinct human interleukin-1 complementary DNAs. Nature 315: 641–647, 1985
Martin, S. A. M., Taylor, B. A., Watanabe, T., and Bulfield, G.: Histidine decarboxylase phenotypes of inbred mouse strains: A regulatory locus (Hdc) determines kidney enzyme concentration. Biochem. Genet. 22: 305–322, 1984
Michaelson, J.: Genetics of beta-2 microglobulin in the mouse. Immunogenetics 17: 219–259, 1983
Mizel, S. B.: Physicochemical characterization of lymphocyte-activating factor (LAF). J. Immunol. 122: 2167–2172, 1979
Rigby, P., Dieckman, M., Rhodes, C., and Berg, P.: Labeling of deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase. J. Mol. Biol. 113: 237–251, 1977
Robinson, P. J., Lundin, L., Sege, K., Graf, L., Wigzell, H., and Peterson, P. A.: Location of the mouse β 2-microglobulin gene B2m determined by linkage analysis. Immunogenetics 14: 449–452, 1981
Silver, J.: Confidence limits for estimates of gene linkage based on analysis of recombinant inbred strains. J. Hered. 76: 436–440, 1985
Simon, P. L. and Willoughby, W. F.: The role of subcellular factors in pulmonary immune functions: Physicochemical characterization of two distinct species of lymphocyte activating factors produced by rabbit alveolar macrophages. J. Immunol. 126: 1534–1541, 1981
Taylor, B. A.: Recombinant inbred strains: use in gene mapping. In H. C. Morse III (ed.): Origins of Inbred Mice, pp. 423–438, Academic Press, New York, 1978
Taylor, B. A.: Recombinant inbred strains. In M. C. Green (ed.): Genetic Variants and Strains of the Laboratory Mouse, pp. 397–407, Gustav Fischer, Stuttgart, 1981
Webb, A. C., Collins, K. L., Auron, P. E., Eddy, R. L., Nakai, H., Byers, M. G., Haley, L. L., Henry, W. M., and Shows, T. B.: Interleukin-1 gene (IL1) assigned to long arm of human chromosome 2. Lymphokine Res. 5: 77–85, 1986
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D'Eustachio, P., Jadidi, S., Fuhlbrigge, R.C. et al. Interleukin-1 α and β genes: linkage on chromosome 2 in the mouse. Immunogenetics 26, 339–343 (1987). https://doi.org/10.1007/BF00343701
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DOI: https://doi.org/10.1007/BF00343701