Abstract
The HLA class I antigen B44 is found in each of two different extended major histocompatibility haplotypes (allele combinations of HLA-B, HLA-DR, and complement genes BF, C2, C4A, and C4B in linkage disequilibrium). Using isoelectric focusing, two variants of HLA-B44 were identified. The basic variant was found in all cell lines with the extended haplotype HLA-B44, DR7, FC31, and the acidic variant in all cell lines with the extended haplotype HLA-B44, DR4, SC30. The occurrence of each antigen variant with a unique extended haplotype explains previous observations concerning the nonrandom association of B44 variants with DR antigens.
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Alper, C. A., Awdeh, Z. L., Raum, D. D., Fleischnick, E., and Yunis, E. J.: Complement genes of the human major histocompatibility complex: Implications for linkage disequilibrium and disease associations. In G. S. Panayi and C. S. David (eds.): Immunogenetics, pp. 50–91, Butterworths, London, 1984
Awdeh, Z. L., Raum, D., Yunis, E. J., and Alper, C. A.: Extended HLA-complement-glyoxalase allele haplotypes: Evidence for T/t-like complex in man. Proc. Natl. Acad. Sci. U. S. A. 80:295–263, 1983
Breur-Vriesendorp, B. S., Neefjes, J. C., Huis, B., van Seventer, G. A., Ploegh, H. L., and Ivanyi, P.: Identification of new B27 subtypes (B27C and B27D) prevalent in oriental populations. Hum. Immunol. 16: 163–168, 1986
Choo, S. Y., Antonelli, P., Nisperos, B., Nepom, G. T., and Hansen, J. A.: Six variants of HLA-B27 identified by isoelectric focusing. Immunogenetics 23: 24–29, 1986
Fleischnick, E., Awdeh, Z. L., Raum, D., Granados, J., Alosco, S. M., Crigler, J. F., Jr., Gerald, P. S., Giles, C. M., Yunis, E. J., and Alper, C. A.: Extended MHC haplotypes in 21-hydroxylase-deficiency congenital adrenal hyperplasia: Shared genotypes in unrelated patients. Lancet 1: 152–156, 1983
Gaston, J. S. H., Rickinson, A. B., and Epstein, M. A.: Epstein-Barr virus-specific cytotoxic T lymphocytes as probes of HLA polymorphism. J. Exp. Med. 158: 280–293, 1983
Gotch, F. M., Kelly, C., Ellis, S. A., Wallace, L., Rickinson, A. B., van der Poel, J., Crumpton, M. J., and McMichael, A. J.: Characterization of the HLA-A2.2 subtype: T cell evidence for further heterogeneity. Immunogenetics 21: 11–23, 1985
Hahn, A. W. A., Schendel, D. J., Hansen, P. W., and Ploegh, H. L.: Biochemical and cellular identification of HLA-Bw44 subtypes. Hum. Immunol. 11: 69–76, 1984
Horai, S., van der Poel, J. J., and Goulmy, E.: Differential recognition of the serologically defined HLA-A2 antigen by allogeneic cytotoxic T cells. Immunogenetics 16: 135–142, 1982
Kato, S., Ivanyi, P., Lacko, E., Breur, B., du Bois, R., and Eijsvoogel, V. P.: Identification of human CML targets: HLA-B locus (B12) antigen variants defined by CTL generated between B locus-identical (B12) responder-stimulator pairs. J. Immunol. 128: 949–955, 1982
Marcus, D. and Alper, C. A.: Methods for allotyping complement proteins. In N. R. Rose, H. Friedman, and J. L. Fahey (eds): Methods in Clinical Immunology, third edition, pp. 185–196, American Society of Microbiology, Washington, DC, 1986
Mölders, H. H., Breuning, M. H., Ivanyi, P., and Ploegh, H. L.: Biochemical analysis of variant HLA-B27 antigen. Hum. Immunol. 6:111–117, 1983
Neefjes, J. J., Breur-Vriesendorp, B. S., van Seventer, G. A., Ivanyi, P., and Ploegh, H. L.: An improved biochemical method for the analysis of HLA-class I antigens: Definition of new HLA-class I subtypes. Hum. Immunol. 16: 169–181, 1986
Raum, D., Awdeh, Z., Yunis, E. J., Alper, C. A., and Gabbay, K. H.: Extended major histocompatibility complex haplotypes in type I diabetes mellitus. J. Clin. Invest. 74: 449–454, 1984
Tekolf, W. A., Biddison, W. E., Aster, R. D., and Shaw, S.: Two subgroups of HLA Bw44 defined by cell-mediated lympholysis that differ in Bw44 expression on platelets and in patterns of genetic linkage disequilibrium. J. Immunol. 129: 1474–1478, 1982
van der Poel, J. J., Mölders, H., Thompson, A., and Ploegh, H. L.: Definition of four HLA-A2 subtypes by CML typing and biochemical analysis. Immunogenetics 17: 609–621, 1983
Vasilov, R. G., Hahn, A., Mölders, H., van Rood, J. J., Breuning, M., and Ploegh, H. L.: Analysis of human class I antigens by two-dimensional gel electrophoresis. I. Polymorphism, evidence for additional (Non-HLA-A, B, C) gene products, and identification of variant HLA-A, B antigens. Immunogenetics 17: 333–356, 1983
Yang, S. Y., Morishima, Y., Collins, N. H., Alton, T., Pollack, M. S., Yunis, E. J., and Dupont, B.: Comparison of one-dimensional IEF patterns for serologically detectable HLA-A and B allotypes. Immunogenetics 19: 217–231, 1984
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Kruskall, M.S., Eynon, E.E., Awdeh, Z. et al. Identification of HLA-B44 subtypes associated with extended MHC haplotypes. Immunogenetics 26, 216–219 (1987). https://doi.org/10.1007/BF00346515
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DOI: https://doi.org/10.1007/BF00346515