Abstract
TL antigens are class I glycoproteins which are expressed on thymocytes and which are coded by the Tla region of the major histocompatibility complex of the mouse. Biochemical analysis of TL molecules from different strains of mice revealed structural variation determined by the Tla region which is detectable by peptide mapping, isoelectric focusing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two-dimensional gels, and by differential reactivity of allelic forms of TL molecules with a panel of anti TL reagents. The quantity of TL expressed on thymocytes is also influenced by the Tla region; three quantitative phenotypes were identified: high (Tla a, Tla d, Tla c), intermediate (Tla c, Tla f), and low (Tla b). (Relative amounts: 1000 : 100 : 1.) Some thymic leukemias arising in (Tla b, Tla c, Tla c) mice with genetically determined reduced levels of thymic TL were found to express TL molecules which were structurally indistinguishable from TL isolated from thymocytes but were present in larger amounts. This suggests that TL structural genes are intrinsically capable of full expression in all mice but that the Tla region of mice expressing an intermediate or low quantity of TL is marked by some feature which causes the thymocyte to express less than the full amount of TL possible.
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Boyse, E. A., Old, L. J., and Stockert, E.: The TL (thymus leukemia) antigen: A review. In P. Grabar and P. A. Miescher (eds.): Immunopathology IV International Symposium, pp. 23–24, Schwaba, Basel, 1965
Ellis, R. W., Stockert, E., and Fleissner, E.: Association of endogenous retroviruses with radiation-induced leukemias in BALB/c mice. J. Virol. 33: 652–660, 1980
Flaherty, L.: Tla-region antigens. In H. Morse (ed.): Origins of Inbred Mice, pp. 33–57, Academic Press, New York, 1978
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970
Lemke, H., Hammerling, G. J., and Hammerling, U.: Fine specificity analysis with monoclonal antibodies of antigens controlled by the major histocompatibility complex and by the Qa/TL region in mice. Immunol. Rev. 47: 175–206, 1979
O'Farrell, P. H.: High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007–4012, 1975
Old, L. J. and Stockert, E.: Immunogenetics of cell surface antigens of mouse leukemia. Annu. Rev. Genet. 11: 127–160, 1977
Michaelson, J.: The genetics of beta-2 microglobulin in the mouse. Immunogenetics 17: 219–259, 1983
Michaelson, J., Flaherty, L., Bushkin, Y., and Yudkowitz, H.: Further biochemical data on Qa-2. Immunogenetics 14: 129–140, 1981
Michaelson, J., Flaherty, L., Hutchinson, B., and Yudkowitz, H.: Qa-2 does not display structural genetic polymorphism detectable on isoelectric-focusing gels. Immunogenetics 16: 363–366, 1982a
Michaelson, J., Tung, J. S., Flaherty, L., Hammerling, U., and Bushking, Y.: The biochemical genetics of TL antigens on mouse thymocytes. Eur. J. Immunol. 12: 257–261, 1982b
Michaelson, J., Boyse, E. A., Chorney, M., Flaherty, L., Fleissner, E., Hammerling, U., Reinisch, C., Rosenson, R., and Shen, E.: The biochemical genetics of the Qa-Tla a region. Transplant. Proc. 15: 2033–2038, 1983
Rothenberg, E. and Boyse, E. A.: Synthesis and processing of molecules bearing thymus leukemia antigen. J. Exp. Med. 150: 777–791, 1979
Shen, F. W., Chorney, M. J., and Boyse, E. A.: Further polymorphism of the 77a locus defined by monoclonal Tla antibodies. Immunogenetics 15: 573–578, 1982
Vitetta, E. S., Uhr, J. W., and Boyse, E. A.: Association of a B2-microglobulin-like subunit with H-2 and TL alloantigens on murine thymocytes. J. Immunol. 114: 252–254, 1975
Yokoyama, K., Stockert, E., Old, L. J., and Nathenson, S. G.: Structural comparisons of TL antigens derived from normal and leukemia cells of TL+ and TL− strains and relationship to genetically linked H-2 major histocompatibility complex products. Proc. Natl. Acad. Sci. U.S.A. 78: 7078–7082, 1982
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Michaelson, J., Boyse, E.A., Ciccia, L. et al. Biochemical genetics of TL antigens. Immunogenetics 24, 103–114 (1986). https://doi.org/10.1007/BF00373117
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DOI: https://doi.org/10.1007/BF00373117