Abstract
Four human T lymphocyte clones exhibiting proliferative responses to class I HLA antigens were isolated from an in vitro mixed lymphocyte culture (MLC). Three clones expressed the Leu-2+3− phenotype and demonstrated proliferation in response to HLA-B8, while the fourth clone expressed the Leu-2−3+ phenotype and proliferated in response to HLA-A2. These clones were also cytotoxic towards cells bearing the same target antigens. Blocking studies utilizing monoclonal antibodies demonstrated that proliferation was triggered by determinants on the class I molecule itself, and these determinants appear to be spatially close to those which determine serologic allospecificity. These findings support the concept that the class I molecules themselves are the weak MLC stimulating determinants previously mapped to the HLA-A and B regions of the major histocompatibility complex.
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Abbreviations
- B-LCL:
-
B-lymphoblastoid cell line
- cpm:
-
counts per minute
- FCS:
-
fetal calf serum
- HS:
-
human serum
- 3H-TdR:
-
tritiated thymidine
- IL-2:
-
interleukin-2
- IL2-CM:
-
interleukin-2 containing conditioned medium
- MHC:
-
major histocompatibility complex
- MLC:
-
mixed lymphocyte culture
- MoAb:
-
monoclonal antibody
- PBL:
-
peripheral blood mononuclear leukocytes
- PLT:
-
primed lymphocyte test
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Flomenberg, N., Russo, C., Ferrone, S. et al. HLA class l specific t lymphocyte clones with dual alloreactive functions. Immunogenetics 19, 39–51 (1984). https://doi.org/10.1007/BF00364474
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DOI: https://doi.org/10.1007/BF00364474