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Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6

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Abstract

Various murine tumor lines were screened by FACS analysis for the surface antigens ThB and Ly-6.2. Positive cell lines were used for immunoprecipitation studies. A monoclonal ThB-specific antibody immunoprecipitated a unique acidic protein of approximately 16 000 daltons from several positive tumors and from concanavalin A (Con-A) and LPS activated splenic lymphocytes. Monoclonal Ly-6.2-specific antibody was used to immunoprecipitate a 33 500 dalton protein that was shown to exist in four similarly sized forms with different basic charges. In the course of these studies, the apparent molecular weight of the surface antigen T 30, immunoprecipitated with a monoclonal T 30-specific antibody from the cell line EL4, was found to be approximately 25 000 daltons.

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Author notes

  1. Arpi Matossian-Rogers

    Present address: Department of Immunology, The London Hospital Medical College, Turner Street, E1. 2AD, London, England

  2. P. Rogers

    Present address: North East London Polytechnic, Romford Road, E15 4LZ, Stratford, London, England

Authors and Affiliations

  1. Department of Genetics, Stanford University School of Medicine, 94305, Stanford, California

    Arpi Matossian-Rogers, P. Rogers, J. A. Ledbetter & L. A. Herzenberg

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  1. Arpi Matossian-Rogers
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  2. P. Rogers
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  3. J. A. Ledbetter
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  4. L. A. Herzenberg
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Matossian-Rogers, A., Rogers, P., Ledbetter, J.A. et al. Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6. Immunogenetics 15, 591–599 (1982). https://doi.org/10.1007/BF00347053

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  • DOI: https://doi.org/10.1007/BF00347053

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