Abstract
Cytotoxic antisera were prepared by immunizing wild-type recipient mice with sperm from donors carrying different recessive lethal alleles at theT locus (T/t 0,T/t w 1,T/t w 5, andT/t w 32). After removal of sperm autoantibody by absorption with sperm of recipient type, each antiserum reacted only with sperm from males whose genotype contained at least one of the immunizing alleles. Cytotoxicity was high against sperm populations in which both immunizing alleles were represented and was lower when only one was present. Thus, each allele at theT locus which has so far been tested serologically is recognizable as a discrete antigen on the surface of sperm.
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This paper is dedicated to Professor Hans Grüneberg, F.R.S. on the occasion of his retirement from the chair of Animal Genetics at University College London, in appreciation of the fact that it was he who first suggested that surface properties played an important role in the origin of abnormalities associated with mutants at theT-locus.
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Yanagisawa, K., Bennett, D., Boyse, E.A. et al. Serological identification of sperm antigens specified by lethalt-alleles in the mouse. Immunogenetics 1, 57–67 (1974). https://doi.org/10.1007/BF01564046
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DOI: https://doi.org/10.1007/BF01564046