Abstract
A retrovirus element (TLev1) is located within the Thymus leukemia antigen (T7a) locus of the C57BL/10 mouse major histocompatibility complex. Low-copy probes have been isolated from sequences flanking the TLev1 integration site to examine the distribution of TLev1 among inbred mouse strains having genotypically determined variations in TL-antigen expression. It was found that the low-copy probes cross-hybridize to regions within the Tla locus in a genotype-specific manner. Although a strong association was found between TL mouse strains and TLev1, the presence or absence of the TLev1 locus did not exclusively correlate with expression or nonexpression of TL antigens. Analysis of different Mus subspecies indicates that TLev1 integrated into a common ancestor of the species Mus musculus. It is suggested that the loss of the TLev1 locus from certain mouse genomes reflects evolutionary rearrangements in the TL region; the resulting diversity may relate to the differential expression of TL antigens among mouse strains. The probes described here provide a useful tool for examining the genomic expansions and contractions which have occurred during the evolution of the Tla locus
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boyse, E. A.: The biology of Tla. Cell 38: 1–2, 1984
Boyse, E. A. and Old, L. J.: Some aspects of normal and abnormal cell surface genetics. Annu. Rev. Genet. 3: 269–290, 1969
Brown, G. D., Choi, Y., Egan, G., and Meruelo, D.: Extension of the H-2 TLb molecular map. Isolation and characterization of T13, T14, and T15 from the C57BL/6 mouse. Immunogenetics 27: 239–251, 1988
Chen, Y.-T., Obata, Y., Stockert, E., and Old, L. J.: Thymus-leukemia (TL) antigens of the mouse. Analysis of TL mRNA and TL cDNA from TL+ and TL- strains. J. Exp. Med. 162: 1134–1148, 1985
Chorney, M. J., Tung, J. S., Buskin, Y., and Shen, F.-W.: Structural characteristics of Tla products. J. Exp. Med. 162: 781–789, 1985
Copeland, N. G., Hutchison, K. W., and Jenkins, N. A.: Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs. Cell 33: 379–387, 1983
Ferris, S. D., Sage, R. D., Prager, E. M., Ritte, U., and Wilson, A. C.: Mitochondrial DNA evolution in mice. Genetics 105: 681–708, 1983
Fisher, D. A., Hunt, S. W., III, and Hood, L.: Structure of a gene encoding a murine thymus leukemia antigen and organization of Tla genes in the BALB/c mouse. J. Exp. Med. 162: 528–545, 1985
Flaherty, L.: Tla-region antigens. In M. E. Dorf (ed.): Role of Major Histocompatibility Complex in Immunobiology, pp. 35–35, Garland Press, New York, 1981
Flaherty, L., Di Biase, K., Lynes, M. N., Seidman, J. G., Weinberger, O., and Renchik, E. M.: Characterization of a Q subregion gene in the murine major histocompatibility complex. Proc. Natl. Acad. Sci. U.S.A. 82: 1503–1507, 1985
Goodenow, R. S.: Identification of class I genes of the mouse MHC complex by DNA-mediated gene transfer. Nature 300: 231–237, 1982
Hämmerling, U., Ronne, H., Widmark, E., Servenius, B., Denaro, M., Rask, L., and Peterson, P. A.: Gene duplications in the TL regions of the mouse major histocompatibility complex. EMBO J. 4: 1431–1434, 1985
Hood, L., Steinmetz, M., and Mallissen, B.: Genes of the major hi stocompatibility complex of the mouse. Annu. Rev. Immunol. 1: 529–568, 1983
Klein, J.: The major histocompatibility complex of the mouse. Science 203: 516–521, 1979
Klein, J., Figueroa, F., and Klein, D.: H-2 haplotypes, genes, and antigens: second listing. 1. Non-H-2 loci on chromosome 17. Immunogenetics 16: 285–317, 1982
Lew, A. M., Lillehoj, E. P., Cowan, E. P., Maloy, W. L., Van Schravendijk, M. R., and Coligan, J. E.: Class I genes and molecules: an update. Immunology 57: 3–18, 1986
Matsuura, A., Shen, F.-W., Fisher, D. A., Hood, L., and Boyse, E. A.: Transcripts of Tla genes. Immunogenetics 25: 411–415, 1987
Michaelson, J., Boyse, E. A., Chorney, M., Flaherty, L., Fleissner, E., Hämmerling, U., Reinish, C., Rosenson, R., and Shen, F.-W.: The biochemical genetics of the Qa-Tla region. Transplant. Proc. 15: 2033–2038, 1983
Morse, H. C., III: Origins of Inbred Mice, pp. 3–21, Academic Press, New York, 1978
Old, L. J. and Stockert, E.: Immunogenetics of cell surface antigens of mouse leukemias. Annu. Rev. Genet. 11: 127–160, 1977
O'Neill, A. E., Reid, K., Garberi, J. C., Karl, M., and Flaherty, L.: Extensive deletions in the Q region of the mouse major histocompatibility complex. Immunogenetics 24: 368–373, 1986
Pampeno, C. L. and Meruelo, D.: Isolation of a retroviruslike sequence from the TL locus of the C57BL/10 murine major histocompatibility complex. J. Virol. 58: 296–306, 1986
Passmore, H. C. and Romano, J.: Genetic organization of the Qa and Tla regions. Gene mapping based on the analysis of recombinant strains. In C. David (ed.): Proceedings of NATO ASI on H-2 Genes, Products and Function, Plenum Publishing Co., New York, 1987
Pontarotti, P. A., Mashimo, H., Zeff, R. A., Fisher, D. A., Hood, L., Mellor, A., Flavell, R. A., and Nathenson, S. G.: Conservation and diversity in the class I genes of the major histocompatibility complex: sequence analysis of a Tla b gene and comparison with a Tla c gene. Proc. Natl. Acad. Sci. U.S.A. 83: 1782–1786, 1986
Rodgers, J. H.: Family organization of mouse H-2 class I genes. Immunogenetics 21: 343–353, 1985
Rotman, G., Itin, A., and Keshet, E.: “Solo” large terminal repeats (LTR) of an endogenous retrovirus-like gene family (VL30) in the mouse genome. Nucleic Acids Res. 12: 2273–2282, 1984
Steinmetz, M., Winoto, A., Minard, K., and Hood, L.: Clusters of genes encoding mouse transplantation antigens. Cell 28: 489–498, 1982
Weiss, E. H., Golden, L., Fahrner, K., Mellor, A. L., Devlin, J. J., Bullman, H., Tiddens, H., Bud, H., and Flavell, R. A.: Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse. Nature 310: 650–655, 1984
Yokoyama, K., Stockert, E., Pease, L. R., Obata, Y., Old, L. J., and Nathenson, S. G.: Polymorphism and diversity in the Tla gene system. Immunogenetics 18: 445–451, 1983
Yonekawa, H., Moriwaki, K., Gotoh, O., Hayashi, J. I., Wantanabe, J., Miyashita, N., Petras, M. L., and Tagashira, Y.: Evolutionary relationships among 5 subspecies of Mus musculus based on restriction enzyme cleavage patterns of mitochondrial DNA. Genetics 98: 801–816, 1981
Zinkernagel, R. M. and Doherty, P. C.: Immunological surveillance against altered self components by sensitized T lymphocytes on lymphocytic choriomeningitis. Nature 251: 547–548, 1974
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pampeno, C., Meruelo, D. Genomic organization of the mouse Tla locus: study of an endogenous retrovirus-like locus reveals polymorphisms related to different Tla haplotypes. Immunogenetics 28, 247–254 (1988). https://doi.org/10.1007/BF00345501
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00345501