Summary
An inverse relationship between proteolytic activity in the presence of kallikrein 0.4 IU and urinary alpha1-antitrypsin concentration has been demonstrated. This protease inhibitor can directly inactivate kallikrein activity. The inhibition was abolished by removal of urinary alpha1-antitrypsin by trypsinsepharose treatment. Inhibition could be reversed by addition of purified alpha1-antitrypsin. These effects could not be demonstrated with inter-alpha-trypsin inhibitor or alpha2-macroglobulin. The inhibitory effect of alpha1-antitrypsin on kallikrein activity should be taken into account in studies in which kallikrein activity is estimated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dahlmann B, Reinauer H (1978) Purification and some properties of an alkaline proteinase from rat skeletal muscle. Biochem J 171: 803–810
Geiger R, Mann K (1976) A kallikrein-specific inhibitor in rat kidney tubules. Hoppe-Seyler's Z Physiol Chem 357: 553–558
Geiger R, Stuckstedte U, Clausnitzer B, Fritz H (1981) Progressive inhibition of glandular (urinary) kallikrein by human serum and identification of the progressive antikallikrein as α1-antitrypsin (α1-protease inhibitor) Hoppe Seyler's Z Physiol Chem 262: 317–325
Harpel PC (1970) Human plasma alpha2-macroglobulin an inhibitor of plasma kallikrein. J Exp Med 132: 329–352
Hojima Y, Yamashita M, Ochi N, Moriwaki C, Moriya H (1977) Isolation and some properties of dog and rat pancreatic kallikreins. Biochem J 81: 599–610
Hörl WH, Heidland A (1981) Inactivation of urinary kallikrein by alpha1-antitrypsin. Klin Wochenschr 59: 761–763
Hörl WH, Stepinski J, Gantert C, Hörl M, Heidland A (1981) Evidence for the participation of proteases on protein catabolism during hypercatabolic renal failure. Klin Wochensch 59: 751–759
Mancini G, Carbonara AO, Heremans JF (1965) Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2: 235–254
Margolius HS, Geller R, Pisano JJ, Sjoerdsma A (1971) Altered urinary kallikrein excretion in human hypertension. Lancet 2: 1063–1065
Margolius HS, Geller R, De Jong W, Pisano JJ, Sjoerdsma A (1972) Altered urinary kallikrein excretion in rats with hypertension. Circ Res 30: 358–362
Mersey JH, Williams GH, Emanuel R, Denhy RG, Wong PW, More JJ (1979) Plasma bradykinin levels and urinary kallikrein excretion in normal renin essential hypertension. J Clin Endocrinol Metab 48: 642–647
Mitas JA, Levy SB, Holle R, Frigon RP, Stone RA (1978) Urinary kallikrein activity in the hypertension of renal parenchymal disease. N Engl J Med 299: 162–165
Oza N, Lieberthal W, Bernard D, Levinsky N (1981) Antibody that recognizes total human urinary kallikrein: radioimmunological determination of inactive kallikrein. J Immunol 126: 2361–2364
Sakamoto W, Nishikaze O (1980) α1-antitrypsin and α2-macroglobulin do not inhibit the kinin-releasing activity of kallikreins from human urine and saliva. Biochim Biophys Acta 633: 305–309
Vahtera E, Hamberg V (1976) Absence of binding of pancreatic and urinary kallikreins to α2-macroglobulin. J Biochem 157: 521–524
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hörl, W.H., Schäfer, R.M. & Heidland, A. Role of urinary alpha1-antitrypsin in padutin® (kallikrein) inactivation. Eur J Clin Pharmacol 22, 541–544 (1982). https://doi.org/10.1007/BF00609628
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00609628