Abstract
Short- and long-term, light-dark, time-course studies of radiocarbon accumulation in the major intracellular end-products of photosynthesis (proteins, polysaccharides, lipids, small metabolites) and extracellular monomers and polymers were conducted at natural light intensity in Belgian coastal waters and in the English Channel on Phaeocystis poucheti colonies growing under depleted and non-limited, inorganic nitrogen concentration. Evidence is given that the exopolymeric substances which compose the colony envelope, massively secreted during the photoperiod, are used during the dark, together with the intracellular reserve products, to cover the carbon and energetic needs of the colonies either for the maintenance or for pursuing protein synthesis, according to the external inorganic nitrogen level.
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Armstrong, F. a., J. Stems and J. D. H. Strickland: The measurement of upwelling and subsequent biological processes by means of the Technicon Autoanalyzer. Deep Sea Res. 14, 381–389 (1976)
Boney, A. D.: Mucilage: the ubiquitous algal attribute. Br. phycol. J. 16, 115–132 (1981)
Burkill, P. H.: Ciliates and other microplankton components of a nearshore food-web: standing stocks and production processes. A. Inst. Oceanogr. Paris 58, 335–350 (1982)
Capriulo, G. M. and E. J. Carpenter: Grazing by 35 to 202 μm micro-zooplankton in Long Island Sound. Mar. Biol. 56, 319–326 (1980)
Gargas, E.: Nitrogen and phosphorus as growth limiting factors in a shallow fjord system. Vatten, 1, 56–63 (1975)
Goldman, J. C.: Identification of nitrogen as a growth limiting nutrient in waste waters and coastal marine waters through continuous culture algal assays. Water Res. 10, 97–104 (1976)
Heinbokel, J. F.: Studies on the functional role of Tintinnids in the Southern California Bight II. Grazing rates of field populations. Mar. Biol. 47, 191–197 (1978)
Joiris, C., G. Billen, C. Lancelot, M. H. Daro, J. P. Mommaerts, A. Bertels, M. Bossicart and J. Nijs: A budget of carbon cycling in the Belgian coastal zone: relative roles of zooplankton, bacterioplankton and benthos in the utilization of primary production. Neth. J. Sea Res. 16, 260–275 (1982)
Lancelot, C.: Factors affecting phytoplankton extracellular release in the Southern Bight of the North Sea. Mar. Ecol. Prog. Ser. 12, 115–121 (1983)
Lancelot, C.: Metabolic changes in Phaeocystis poucheti (Hariot) Lagerheim during the spring bloom in Belgian coastal waters. Estuar. cstl Shelf Sci. 18, 593–600 (1984)
Lancelot, C. and G. Billen: Activity of heterotrophic bacteria and the coupling to primary production during the spring phytoplankton bloom in the Southern Bight of the North Sea. Limnol. Oceanogr. 29, 721–730 (1984)
Lancelot, C. and G. Billen: Carbon-nitrogen relationship in nutrient metabolism of coastal marine ecosystems. Adv. aquat. Microbiol. (In press)
Lancelot, C. and S. Mathot: Biochemical tractionation of primary production by phytoplankton in Belgian coastal waters during short- and long-term incubations with 14C-bicarbonate I. Mixed diatom population. Mar. Biol. 86, 219–226 (1985)
Parsons, T. R., M. Takahashi and B. Hargrave: Biological oceanographic processes, pp 105–111, 2nd ed. Oxford: Pergamon Press 1977
Ryther, J. H. and W. M. Dunstan: Nitrogen, phosphorus and eutrophication in the coastal marine environment. Science, N. Y. 171, 1008–1013 (1971)
Sakshaug, E. and S. Myklestad: Studies on the phytoplankton ecology of the Trondheimsfjord. 3. Dynamics of phytoplankton blooms in relation to environmental factors, bioassay experiments and parameters for the physiological state of populations. J exp. mar. Biol. Ecol. 11, 157–188 (1973)
Smayda, T. J.: Bioassay on the growth potential of the surface water of lower Narragansett Bay over an annual cycle using the diatom Thalassiosira pseudonana (Oceanic clone 13-1). Limnol. Oceanogr. 19, 889–901 (1974)
Slawyk, G. and J. J. McIsaac: Comparison of two automated ammonium methods in a region of coastal upwelling. Deep Sea Res. 19, 1–4 (1972)
Thomas, W.H.: Phytoplankton nutrient enrichment experiments off Baja (California) and in the eastern equatorial Pacific Ocean. J. Fish. Res. Bd Can. 26, 1133–1145 (1969)
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Communicated by D. Kinne, Oldendorf/Luhe
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Lancelot, C., Mathot, S. Biochemical fractionation of primary production by phytoplankton in Belgian coastal waters during short- and long-term incubations with 14C-bicarbonate. Mar. Biol. 86, 227–232 (1985). https://doi.org/10.1007/BF00397508
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DOI: https://doi.org/10.1007/BF00397508