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Determination of 3-amino-1,2,4-triazole (Amitrole) in tissues with electrochemical detection by ion-pair high-performance liquid chromatography

Bestimmung von 3-Amino-1,2,4-triazol (Amitrol) in tierischen Matrices mit elektrochemischer Detektion und Ionenpaar-Hochdruckflüssigkeits-Chromatographie

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Zusammenfassung

Es wird eine hochdruckflüssigkeits-chromatographische Trennmethode an einer RP-18-Säule ohne Derivatisierung mit elektrochemischer Detektion beschrieben. Die Probenaufarbeitung erfolgt mit einer Mini-Alox-Säule und gel-chromatographischer Reinigung für hydrophile Wirkstoffe. Die Methode erreicht eine Nachweisgrenze von 60 ng/g bei einer Probeneinwaage von 2 g. Die mittlere Wiederfindungsrate beträgt 79,8±4,3% (in Leber).

Summary

A high-performance liquid chromatographical separation method at a RP-18 column without derivatisation and with electrochemical detection is described. The samples are prepared with a mini-Alox-column and a gelchromatographical clean-up for hydrophilic substances. The method obtains a detection limit of 60 ng/g with a sample weight of 2 g. The average recovery is 79.8±4.3% (in liver).

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Authors and Affiliations

  1. Chemisches Institut der Tierärztlichen Hochschule Hannover, Bischofsholer Damm 15, D-3000, Hannover 1, Federal Republic of Germany

    W. Ternes & H. A. Rüssel-Sinn

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  1. W. Ternes
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  2. H. A. Rüssel-Sinn
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Ternes, W., Rüssel-Sinn, H.A. Determination of 3-amino-1,2,4-triazole (Amitrole) in tissues with electrochemical detection by ion-pair high-performance liquid chromatography. Z. Anal. Chem. 326, 757–759 (1987). https://doi.org/10.1007/BF00487371

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  • DOI: https://doi.org/10.1007/BF00487371

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