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HPLC-based measurement of the chelator 1,2-dimethyl-3-hydroxy-pyrid-4-one (L1) and its iron complex for pharmacokinetic studies in humans

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  • Organic Chemistry
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Abstract

An improved HPLC based method to assay the oral active iron chelator 1,2-dimethyl-3-hydroxypyrid-4-one (L1, CP20) in serum and urine is described. The L1 peak has been well separated from other endogenous compounds, allowing the exact determination of the drug in both biological fluids. Moreover urinary iron excretion due to L1 therapy has been monitored by measuring urine Fe-(L1)3 complex concentrations using reverse phase HPLC and subsequent detection at 450 nm. In patients and normal volunteers receiving this drug there is a good correlation between urine iron excretion measured by AAS and by the HPLC based method.

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Authors and Affiliations

  1. Abteilung für Medizinische Biochemie, Institut für Physiologische Chemie, Universitätskrankenhaus Eppendorf, Martinistrasse 52, D-20246, Hamburg, Germany

    Bernd Dresow, Roland Fischer & Erich E. Gabbe

  2. Kinderklinik, Universitätskrankenhaus Eppendorf, Martinistrasse 52, D-20246, Hamburg, Germany

    Gritta E. Janka

Authors
  1. Bernd Dresow
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  2. Roland Fischer
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  3. Gritta E. Janka
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  4. Erich E. Gabbe
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Dresow, B., Fischer, R., Janka, G.E. et al. HPLC-based measurement of the chelator 1,2-dimethyl-3-hydroxy-pyrid-4-one (L1) and its iron complex for pharmacokinetic studies in humans. Fresenius J Anal Chem 352, 562–564 (1995). https://doi.org/10.1007/BF00323074

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  • DOI: https://doi.org/10.1007/BF00323074

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