Summary
The carbohydrate composition and the specific activity of the trehalase of cyclic partially synchronised yeast populations have been investigated. Under glucose limitation and appropriate cultural conditions synchronous growth in a chemostat was achieved. The cells accumulated the reserve carbohydrates during the single cell phase between two buddings. The rapid degradation of part of these reserves began shortly before the swelling of the bud. The importance of the mobilisation of endogenous reserves for the development of the cell is discussed.
The specific activity of the trehalase changed during the budding cycle. The result gives rise to the assumption that the synthesis of this enzyme is linked to the growth cycle.
Similar content being viewed by others
References
Beck, C., and H. K. von Meyenburg: Enzyme pattern and aerobic growth of Saccharomyces cerevisiae under various degrees of glucose limitation. J. Bact. 96, 479–486 (1968).
Berke, H. L., and A. Rothstein: The metabolism of storage carbohydrates in yeast, studied with glucose-1-C14 and dinitrophenol. Arch. Biochem. 72, 380–395 (1957).
Brandt, K. M.: Über die Reservekohlehydrate der Preßhefe. Das Verhalten der Trehalose beim Wachstum und bei der Wärmeschädigung der Hefe. Biochem. Z. 309, 190–201 (1941).
Ceccarini, C.: The biochemical relationship between trehalase and trehalose during growth and differentiation in the cellular slime mold, Dictyostelium discoideum. Biochim. biophys. Acta (Amst.) 148, 114–124 (1967).
Chester, V. E.: The dissimilation of the carbohydrate reserves of a strain of Saccharomyces cerevisiae. Biochem. J. 86, 153–160 (1963).
—: Comparative studies on the dissimilation of reserve carbohydrate in four strains of Saccharomyces cerevisiae. Biochem. J. 92, 318–323 (1964).
Eaton, R. E.: Endogenous respiration of yeast. I. The endogenous substrate. Arch. Biochem. 88, 17–25 (1960).
—: Endogenous metabolism of reserve deposits in yeast. Ann. N. Y. Acad. Sci. 102, 678–687 (1963).
Engelberg, J.: Measurement of degrees of synchrony in cell populations. In: Synchrony in cell division and growth (Ed. E. Zeuthen), pp. 497–508. New York-London-sydney: Interscience Publishers 1964.
Fiechter, A.: Die kontinuierliche Züchtung von Mikroorganismen als apparatives Problem. Biotechnol. Bioeng. 7, 101–128 (1965).
—, and H. K. von Meyenburg: Automatic analysis of gas exchange in microbial systems. Biotechnol. Bioeng. 10, 535 (1968).
Halvorson, H. O., R. M. Bock, P. Tauro, R. Epstein, and M. LaBerge: Periodic enzyme synthesis in synchronous cultures of yeast. In: Cell synchrony (Eds. Cameron, I. L., and G. M. Padilla), pp. 102–116. New York-London: Academic Press 1966.
Krüger, J., and B. Hess: Über zwei Formen von Trehalase in S. carlsbergensis. Arch. Mikrobiol. 61, 154–158 (1968).
Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Meyenburg, H. K. v.: Der Sprossungscyclus von Saccharomyces cerevisiae. Path. et Microbiol. (Basel) 31, 117–127 (1968a).
Meyenburg, H. K. v.: Energetics of the budding of Saccharomyces cerevisiae during glucose limited aerobic growth. Paper presented at the 4th Intern. Symp. on Continuous Cultivation of Microorganism. Prague 1968b.
Mitchison, J. M., and W. S. Vincent: Preparation of synchronous cell cultures by sedimentation. Nature (Lond.) 205, 987–989 (1965).
Monod, J.: La technique de culture continuée. Théorie et application. Ann. Inst. Pasteur 79, 390–410 (1950).
Nelson, N.: A photometric adaptation of the Somogyi method for the determination of glucose. J. biol. Chem. 153, 375–380 (1944).
Northcote, D. H., and R. W. Horne: The chemical composition and structure of the yeast cell wall. Biochem. J. 51, 232–236 (1952).
Panek, A.: Synthesis of trehalose by baker's yeast (Saccharomyces cerevisiae). Arch. Biochem. 98, 349–355 (1962).
—: Function of trehalose in baker's yeast (Saccharomyces cerevisiae). Arch. Biochem. 100, 422–425 (1963).
Somogyi, M.: A new reagent for the determination of sugar. J. biol. Chem. 160, 61–68 (1945).
Souza, N. O., and A. D. Panek: Location of trehalase and trehalose in yeast cells. Arch. Biochem. 125, 22–28 (1968).
Sylvén, B., C. A. Tobias, H. Malmgren, R. Ottoson, and B. Thorell: Cyclic variations in the peptidase and catheptic activities of yeast cultures synchronized with respect to cell multiplication. Exp. Cell Res. 16, 75–87 (1959).
Trevelyan, W. E.: Synthesis and degradation of cellular carbohydrates by yeasts. In: The chemistry and biology of yeasts (Ed. A. H. Cook), pp. 369–436. New York: Academic Press Inc. 1958.
—, and J. S. Harrison: Studies on yeast metabolism. 7. Yeast carbohydrate fractions. Separation from nucleic acid, analysis, and behaviour during anaerobic fermentation. Biochem. J. 63, 23–33 (1956).
Williamson, D. H., and A. W. Scopes: A rapid method for synchronizing division in the yeast, Saccharomyces cerevisiae. Nature (Lond.) 193, 256–257 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Küenzi, M.T., Fiechter, A. Changes in carbohydrate composition and trehalase-activity during the budding cycle of Saccharomyces cerevisiae . Archiv. Mikrobiol. 64, 396–407 (1969). https://doi.org/10.1007/BF00417021
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00417021