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Zur Wirkungsweise zellwandhemmender Antibiotica bei gramnegativen Bakterien

I. Die Wirkung von Penicillin auf die Konzentration von Zellwandvorstufen bei Proteus mirabilis

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Zusammenfassung

Es wurde der Einfluß von Penicillin auf die Konzentrationen der freien Nucleotide in Proteus mirabilis D52 und in dessen instabiler L-Phase untersucht. Zellextrakte wurden an Dowex-1 chromatographiert und ein Teil der dabei isolierten Nucleotide charakterisiert.

  1. 1.

    Mit der durch Penicillin verursachten Umwandlung stäbchenförmiger Proteus-Bakterien in Sphäroplasten ist keine Anreicherung UDP-aktivierter Mucopolymervorstufen verbunden. Lediglich die Konzentration von UMP nimmt dabei stark ab.

  2. 2.

    Sowohl in Proteus D 52 als auch in penicillininduzierten Sphäroplasten von Proteus D 52 konnten geringe Mengen von UDP-aktivierten Mucopolymervorstufen nachgewiesen werden; ihre Konzentration ist in Sphäroplasten nicht höher als in stäbchenförmigen Zellen von Proteus D 52.

  3. 3.

    Die instabile, mucopolymerhaltige L-Phase I Ca von P. mirabilis D 52 weist gegenüber Proleus D 52 und penicillininduzierten Sphäroplasten von Proteus D 52 einen vielfach höheren Gehalt an Cytidinnucleotiden auf. Der Gehalt an Mucopolymervorstufen ist praktisch gleich.

Es wird daher angenommen, daß Penicillin die morphologischen Änderungen, z. B. die Bildung von Sphäroplasten und instabilen l-Formen von P. mirabilis nicht durch die Hemmung der Polymerisation UDP-aktivierter Vorstufen verursacht, sondern durch die Hemmung späterer Schritte der Zellwandsynthese wie z. B. die Verbindung des Mucopolymers mit Lipoprotein.

Summary

The influence of penicillin on the concentration of nucleotide-activated mucopolymer precursors was studied in Proteus mirabilis and in its unstable L-form. Cell extracts were chromatographed on Dowex-1, and some of the nucleotides were characterized.

  1. 1.

    During the penicillin-induced transition of cells of P. mirabilis to spheroplasts no accumulation of UDP-activated mucopolymer precursors was observed. Only the concentration of UMP decreases during the formation of spheroplasts.

  2. 2.

    In P. mirabilis and in its spheroplasts small amounts of mucopolymer precursors were detected their concentration being not higher in spheroplasts than in rod-shaped cells of P. mirabilis.

  3. 3.

    The unstable, mucopolymer-containing L-form I Ca, derived from P. mirabilis by use of penicillin, contains approximately the same amount of mucopolymer precursors as rod-shaped cells of P. mirabilis. The concentration of cytidine nucleotides was many times higher in the L-form than in P. mirabilis.

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Literatur

  • Abraham, E. P.: The antibiotics. In: Comprehensive Biochemistry, Vol. 11, p. 181 to 224, ed. M. Florkin, and E. H. Stotz Amsterdam: Elsevier Publishing Co. 1963.

    Google Scholar 

  • Abrams, R. Y.: A method for the cultivation of l-forms in liquid media. J. Bact. 70, 251 (1955).

    PubMed  Google Scholar 

  • Anwar, R. A., C. Roy, and R. W. Watson: Isolation and structure of uridine nucleotide-peptides from Aerobacter cloacae NRC 492. Canad. J. Biochem. 41, 1065–1072 (1963).

    PubMed  Google Scholar 

  • Boivin, A., J. Mesrobeanu et L. Mesrobeanu: Technique pour la preparation des polysaccharides microbiens spécifiques. C. R. Acad. Sci. (Paris) 113, 490–492 (1933).

    Google Scholar 

  • Brenner, S., F. A., Dark, P. Gerhardt, M. H. Jeynes, O. Kandler, E. Kellenberger, E. Klieneberger-Nobel, K. McQuillen, M. Rubio-Huertos, M. R. J. Salton, R. E. Strange, J. Tomcsik, and C. Weibull: Bacterial protoplasts. Nature (Lond.) 181, 1713–1715 (1958).

    Google Scholar 

  • Caputto, R., L. F. Leloir, C. E. Cardini, and A. C. Paladini: Isolation of the coenzyme of the galactose phosphate-glucose phosphate transformation. J. biol. Chem. 184, 333–350 (1950).

    PubMed  Google Scholar 

  • Comb, D. G., W. Chin, and S. Roseman: Uridine nucleotides containing α, s-diaminopimelic acid from Escherichia coli. Biochim. biophys. Acta (Amst.) 46, 394–397 (1961).

    Article  Google Scholar 

  • Cummins, C. S., and H. Harris: The chemical composition of cell wall in some Gram-positive bacteria and its possible value as a taxonomic character. J. gen. Microbiol. 14, 583–600 (1956).

    PubMed  Google Scholar 

  • Dienes, L.: The development of Proteus cultures in the presence of penicillin. J. Bact. 57, 529–546 (1949).

    Google Scholar 

  • — and H. J. Weinberger: The l forms of bacteria. Bact. Rev. 15, 245–288 (1951).

    PubMed  Google Scholar 

  • Hahn, F. E., and J. Ciak: Penicillin-induced lysis of Escherichia coli. Science 125, 119–120 (1957).

    PubMed  Google Scholar 

  • Hais, J. M., u. K. Macek: Handbuch der Papierchromatographie. Jena: G. Fischer 1958.

    Google Scholar 

  • Hannig, K.: Erfahrungen mit der quantitativen Aminosäurebestimmung an Ionenaustauschersäulen und automatischer Registrierung der Ergebnisse. Clin. chim. Acta 4, 51–57 (1959).

    Article  PubMed  Google Scholar 

  • Hurlbert, R. B., H. Schmitz, A. F. Brumm, and V. R. Potter: Nucleotide metabolism. II. Chromatographic separation of acid-soluble nucleotides. J. biol. Chem. 209, 23–29 (1954).

    PubMed  Google Scholar 

  • Ito, E., and J. L. Strominger: Enzymatic synthesis of the peptide in bacterial uridine nucleotides I. Enzymatic addition of l-Alanine, d-Glutamic acid and l-Lysine. J. biol. Chem. 237, 2689–2695 (1962a).

    Google Scholar 

  • ——: Enzymatic synthesis of the peptide in bacterial uridine nucleotides II. Enzymatic synthesis and addition of d-Alanyl-d-Alanine. J. biol. Chem. 237, 2696–2703 (1962b).

    Google Scholar 

  • Kandler, O., u. G. Kandler: Trennung und Charakterisierung verschiedener L-Phasen-Typen von Proteus vulgaris. Z. Naturforsch. 11b, 252–259 (1956).

    Google Scholar 

  • —, u. C. Zehender: Über das Vorkommen von α,ɛ-Diaminopimelinsäure bei verschiedenen L-Phasentypen von Proteus vulgaris und bei pleuropneumonieähnlichen Organismen. Z. Naturforsch. 12b, 725–728 (1957).

    Google Scholar 

  • —, and C. Zehender: Cell wall composition in bacterial and L forms of Proteus vulgaris. Nature (Lond.) 181, 572–573 (1958a).

    Google Scholar 

  • ———: Eine einfache Methode zur Isolierung der “Membran-Grundsubstanz” grampositiver und gramnegativer Bakterien. Arch. Mikrobiol. 30, 355–362 (1958b).

    PubMed  Google Scholar 

  • —, u. G. Kandler: Die L-Phase der Bakterien. Ergebn. Mikrobiol. 33, 97–127 (1960).

    Google Scholar 

  • Landman O. E., R. A. Altenbern, and H. S. Ginoza: Quantitative conversion of cells and protoplasts of Proteus mirabilis and Escherichia coli to the L-form. J. Bact. 75, 567–576 (1958).

    PubMed  Google Scholar 

  • Lark, C., and K. G. Lark: The effects of d-amino acids on Alcaligenes faecalis. Canad. J. Microbiol. 5, 369–379 (1959).

    Google Scholar 

  • Lederberc, J.: Bacterial protoplasts induced by penicillin. Proc. nat. Acad. Sci. (Wash.) 42, 574–577 (1956).

    Google Scholar 

  • —, and J. St. Clair: Protoplast and L-type growth of Escherichia coli. J. Bact. 75, 143–160 (1958).

    PubMed  Google Scholar 

  • Leutgeb, W., H. Pelzer, u. U. Schwarz: Struktur und Stoffwechsel von E. coli-Stützmembranen. Zbl. Bakt., I. Abt. Orig. 191, 400–408 (1963).

    Google Scholar 

  • Liebermeister, K., u. E. Kellenberger: Studien zur L-Form der Bakterien. I. Die Umwandlung der bazillären in die globuläre Zellform bei Proteus unter Einfluß von Penicillin. Z. Naturforsch. 11b, 200–206 (1956).

    Google Scholar 

  • Martin, H. H.: Composition of the mucopolymer in cell walls of the unstable and stable L-form of Proteus mirabilis. J. gen. Microbiol. 34, 195–202 (1964).

    PubMed  Google Scholar 

  • Mayer, R. M., and V. Ginsburg: Isolation of cytidine 5′-phosphate paratose from Salmonella paratyphi A. Biochem. biophys. Res. Commun. 15, 334–337 (1964).

    Google Scholar 

  • Meadow, P. M., J. S. Anderson, and J. L. Strominger: Enzymatic polymerization of UDP-acetylmuramyl-(l)-ala-(d)-glu-(l)-lys-(d)-ala-(d)-ala and UDP-acetylglucosamine by a particulate enzyme from Staphylococcus aureus and its inhibition by antibiotics. Biochem. biophys. Res. Commun. 14, 382–387 (1964).

    PubMed  Google Scholar 

  • Morgan, W. T. J., and L. A. Elson: A colorimetric method for the determination of n-acetylglucosamine and n-acetylchondrosamine. Biochem. J. 28, 988–995 (1934).

    Google Scholar 

  • Morrison, H. T., and C. Weibull: The occurrence of cell wall constituents in stable Proteus L forms. Acta path. microbiol. scand. 55, 475–482 (1962).

    PubMed  Google Scholar 

  • Nikaido, H., and K. Jokura: Isolation of cytidine diphosphate 3,6-dideoxyhexoses from Salmonella. Biochem. biophys. Res. Commun. 6, 304–309 (1961).

    PubMed  Google Scholar 

  • Okabayashi, T.: Occurrence of nucleotides in culture fluids of microorganisms. III. Nucleotide excretion by Brevibacterium liquefaciens sp. n. with special reference to uridine diphospho-n-acetylglucosamine compounds. J. Bact. 84, 1–8 (1962).

    PubMed  Google Scholar 

  • Okuda, S., N. Suzuki, and S. Suzuki: Occurrence of CDP-sugar compounds in Azotobacter vinelandii. Biochim. biophys. Acta (Amst.) 82, 436–438 (1964).

    Google Scholar 

  • Park, J. T., and M. J. Johnson: Accumulation of labile phosphate in Staphylococcus aureus grown in the presence of penicillin. J. biol. Chem. 179, 585–592 (1949).

    Google Scholar 

  • Park, J. T., and M. J. Johnson: Uridine-5′-pyrophosphate derivatives. I. Isolation from Staphylococcus aureus. J. biol. Chem. 194, 877–884 (1952).

    PubMed  Google Scholar 

  • —: Uridine-5′-pyrophosphate derivatives. II. Astructure common to three derivatives. J. biol. Chem. 194, 885–895 (1952).

    PubMed  Google Scholar 

  • —: III. Amino acid-containing derivatives. J. biol. Chem. 194, 897–904 (1952).

    PubMed  Google Scholar 

  • —, and J. L. Strominger: Mode of action of penicillin. Science 125, 99–101 (1957).

    PubMed  Google Scholar 

  • Pease, Ph.: The electronmicroscopy of L-forms induced by penicillin in Proteus vulgaris. J. gen. Microbiol. 17, 64–67 (1957).

    PubMed  Google Scholar 

  • Perkins, H. R.: Chemical structure and biosynthesis of bacterial cell walls. Bact. Rev. 27, 18–55 (1963).

    PubMed  Google Scholar 

  • Pontis, N. G., E. Cabib, and L. F. Leloir: An improved method for the isolation of some nucleoside diphosphate sugars from yeast. Biochim. biophys. Acta (Amst.) 26, 146–150 (1957).

    Google Scholar 

  • Reissig, J. L., J. L. Strominger, and L. F. Leloir: A modified colorimetric method for the estimation of n-acetyl-amino sugars. J. biol. Chem. 217, 959–966 (1955).

    PubMed  Google Scholar 

  • Schmidt, W. H., and A. J. Moyer: Penicillin. I. Methods of assay. J. Bact. 47, 199–210 (1944).

    Google Scholar 

  • Spackman, D. H., W. H. Stein, and S. Moore: Automatic recording apparatus for use in chromatography of amino acids. Analyt. Chem. 30, 1190–1205 (1958).

    Google Scholar 

  • Stempen, H.: Demonstration of a cell wall in the large bodies of Proteus vulgaris. J. Bact. 70, 177–181 (1955).

    PubMed  Google Scholar 

  • Strominger, J. L.: Microbiol. uridine-5′-pyrophosphate n-acetyl-amino sugar compounds. I. Biology of the penicillin-induced accumulation. J. biol. Chem. 224, 509–523 (1957).

    PubMed  Google Scholar 

  • —: The amino acid sequence in the uridine nucleotidepeptide from Staphylococcus aureus. C. R. Lab. Carlsberg 31, 181–182 (1959b).

    Google Scholar 

  • —: Accumulation of uridine and cytidine nucleotides in Staphylococcus aureus inhibited by gentian violet. J. biol. Chem. 234, 1520–1524 (1959e).

    PubMed  Google Scholar 

  • —: Biosynthesis of bacterial cell walls. In: The Bacteria, Vol. III, p. 413–470. Hrsg. von I. C. Gunsalus und Y. Stanier. New York u. London: Academic Press 1962.

    Google Scholar 

  • —, and R. E. Thompson: Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin. J. biol. Chem. 234, 3263–3268 (1959c).

    PubMed  Google Scholar 

  • —, and R. H. Threnn: Isolation from E. coli of a uridine nucleotide containing diaminopimelic acid. Fed. Proc. 18, 334 (1959d).

    Google Scholar 

  • —, and R. H. Threnn: The optical configuration of the alanine residues in a uridine nucleotide and in the cell wall of Staphylococcus aureus. Biochim. biophys. Acta (Amst.) 33, 280–281 (1959a).

    Google Scholar 

  • Taubeneck, U.: Über Plasmolyseversuche mit penicillininduzierten Umbildungsformen von Proteus vulgaris. Zbl. Bakt., I. Abt. Orig. 163, 477–483 (1955).

    Google Scholar 

  • —: Die Penicillininaktiverung durch Proteus-Arten. Zbl. Bakt., I. Abt. Orig. 167, 345–346 (1956a).

    Google Scholar 

  • —: Über die Inaktivierung von Penicillin durch Proteus vulgaris. Arch. Mikrobiol. 24, 423–436 (1956b).

    PubMed  Google Scholar 

  • —: Untersuchungen über die L-Phase von Proteus mirabilis mit Hilfe von Bakteriophagen. Biol. Zbl. 77, 663–673 (1958b).

    Google Scholar 

  • —: Die Phagenresistenz der stabilen L-form von Proteus mirabilis. Z. Naturforsch. 16b, 849 (1961).

    Google Scholar 

  • —, u. H. Böhme: Der Einfluß von Bakteriophagen auf die L-Phase. Z. Naturforsch. 13b, 471–472 (1958a).

    Google Scholar 

  • Tulasne, R., R. Minck, A. Kirn et J. Krembel: Délimination de la notion des formes l des bactéries: protoplastes, sphéroplastes et formes L. Ann. Inst. Pasteur 99, 859–874 (1960).

    Google Scholar 

  • Weibull, C.: The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme.

  • —: Bacterial protoplasts. Ann. Rev. Microbiol. 12, 1–26 (1958a).

    Google Scholar 

  • —: Chemical analysis elucidating the structure of bacterial L forms. Acta path. microbiol. scand. 42, 324 (1958b).

    PubMed  Google Scholar 

  • Weidel, W., H. Frank, and H. H. Martin: The rigid layer of the cell wall of Escherichia coli strain B. J. gen. Microbiol. 22, 158–166 (1960).

    PubMed  Google Scholar 

  • —, u. J. Primosigh: Die gemeinsame Wurzel der Lyse von Escherichia coli durch Penicillin oder durch Phagen. Z. Naturforsch. 12b, 421–427 (1957).

    Google Scholar 

  • Westphal, O., u. O. Lüderitz: Chemische Erforschung von Lipopolysacchariden gramnegativer Bakterien. Angew. Chem. 66, 407–417 (1954).

    Google Scholar 

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Plapp, R., Kandler, O. Zur Wirkungsweise zellwandhemmender Antibiotica bei gramnegativen Bakterien. Archiv. Mikrobiol. 50, 171–193 (1965). https://doi.org/10.1007/BF00409127

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  • DOI: https://doi.org/10.1007/BF00409127

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