Skip to main content
SpringerLink
Log in

Southern blotting analyses of strains ofCampylobacter fetus using the conserved region ofsapA

  • Short Communication
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Chromosomal DNA of 27 strains ofCampylobacter fetus was analyzed by Southern blotting with a probe of the conserved region ofsapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than sixsapA homologs. In Southern blots ofSalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiplesapA homologs are localized to a limited region on the chromosomal DNA ofC. fetus and thus suggest the possibility of developing a typing system using this method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

PCR :

Polymerase chain reaction

PFGE :

Pulsed-field gel electrophoresis

LPS :

Lipopolysaccharide

SAP :

Surface array protein

References

  • Blaser MJ (1993) Role of the S-layer proteins ofCampylobacter fetus in serum-resistance and antigenic variation: a model of bacterial pathogenesis. Am J Med Sci 306:325–9

    Article  PubMed  CAS  Google Scholar 

  • Blaser MJ, Pei Z (1993) Pathogenesis ofCampylobacter fetus infections: critical role of high-molecular-weight S-layer proteins in virulence. J Infect Dis 167:372–377

    PubMed  CAS  Google Scholar 

  • Blaser MJ, Gotschlich EC (1990) Surface array protein ofCampylobacter fetus. Cloning and gene structure. J Biol Chem 265: 14529–35

    PubMed  CAS  Google Scholar 

  • Dubreuil JD, Kostrzynska M, Austin JW, Trust TJ (1990) Antigenic differences amongCampylobacter fetus S-layer proteins. J Bacteriol 172:5035–5043

    PubMed  CAS  Google Scholar 

  • Dworkin J, Tummuru MKR, Blaser MJ (1995) A lipopolysaccharide-binding domain of theCampylobacter fetus S-layer protein resides within the conserved N terminus of a family of silent and divergent homologs. J Bacteriol 177:1734–1741

    PubMed  CAS  Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    Article  PubMed  CAS  Google Scholar 

  • Fujimoto S, Takade A, Amako K, Blaser MJ (1991) Correlation between molecular size of the surface array protein and morphology and antigenicity of theCampylobacter fetus S layer. Infect Immun 59:2017–22

    PubMed  CAS  Google Scholar 

  • Fujita M, Amako K (1994) Localization of thesapA gene on the physical map ofCampylobacter fetus. Arch Microbiol 162: 375–380

    PubMed  CAS  Google Scholar 

  • Fujita M, Fujimoto S, Morooka T, Amako K (1995) Analysis of the strains ofCampylobacter fetus by pulsed-field gel electrophoresis. J Clin Microbiol 33:1676–1678

    PubMed  CAS  Google Scholar 

  • Höltke HJ, Sagner G, Kessler C, Schmitz G (1992) Sensitive chemiluminescent detection of digoxigenin-labeled nucleic acids: a fast and simple protocol and its applications. Biotechniques 12:104–113

    PubMed  Google Scholar 

  • Messner P, Sleytr UB (1992) Crystalline bacterial cell-surface layers. Adv Microb Physiol 33:213–275

    PubMed  CAS  Google Scholar 

  • Morooka T, Takeo H, Takeshita S, Mimatsu T, Yukitake K, Oda T (1988) Nosocomial meningitis due toCampylobacter fetus subsp.fetus in a neonatal intensive care unit. Eur J Pediatr 148: 89–90

    Article  PubMed  CAS  Google Scholar 

  • Pei Z, Blaser MJ (1990) Pathogenesis ofCampylobacter fetus infections. J Clin Invest 85:1036–1043

    Article  PubMed  CAS  Google Scholar 

  • Perez-Perez GI, Blazer MJ, Bryner JH (1986) Lipopolysaccharide structures ofCampylobacter fetus are related to heat-stable ser-groups. Infect Immun 51:209–212

    PubMed  CAS  Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491

    Article  PubMed  CAS  Google Scholar 

  • Smith CL, Econome JG, Schutt A, Klco S, Cantor CR (1987) A physical map of theEscherichia coli K12 Genome. Science 236:1448–1453

    Article  PubMed  CAS  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Article  PubMed  CAS  Google Scholar 

  • Tummuru MKR, Blaser MJ (1992) Characterization of theCampylobacter fetus sapA promoter: evidence that thesapA promoter is deleted in spontaneous mutant strains. J Bacteriol 174:5916–5922

    PubMed  CAS  Google Scholar 

  • Tummuru MKR, Blaser MJ (1993) Rearrangement ofsapA homologs with conserved and variable regions inCampylobacter fetus. Proc Natl Acad Sci USA 90:7265–7269

    Article  PubMed  CAS  Google Scholar 

  • Wang E, Garcia MM, Blake MS, Pei Z, Blaser MJ (1993) Shift in S-layer protein expression responsible for antigenic variation inCampylobacter fetus. J Bacteriol 175:4979–4984

    PubMed  CAS  Google Scholar 

  • Wassenaar TM, Bleumink-Pluym NMC, Van derZeijst BAM (1991) Inactivation ofCampylobacter jejuni flagellin genes by homologous recombination demonstrates thatflaA but notflaB is required for invasion. EMBO J 10:2055–2061

    PubMed  CAS  Google Scholar 

  • Winter AJ, McCoy EC, Fullmer CS, Burda K, Bier PJ (1978) Microcapsule ofCampylobacter fetus: chemical and physical characterization. Infect Immun 22:963–971

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bacteriology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan

    Masaki Fujita, Shuji Fujimoto, Tetsuhiro Moriya & Kazunobu Amako

  2. Department of Pediatrics, Chikushi Hospital, Fukuoka University, Fukuoka, Japan

    Tatsuya Morooka

Authors
  1. Masaki Fujita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tatsuya Morooka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuji Fujimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tetsuhiro Moriya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kazunobu Amako
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masaki Fujita.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujita, M., Morooka, T., Fujimoto, S. et al. Southern blotting analyses of strains ofCampylobacter fetus using the conserved region ofsapA . Arch. Microbiol. 164, 444–447 (1995). https://doi.org/10.1007/BF02529743

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02529743

Key words

Search

Navigation