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Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134

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Abstract

2,4-Dichlorophenoxyacetate (2,4-D) in Alcaligenes eutrophus JMP134 (pJP4) is degraded via 2-chloromaleylacetate as an intermediate. The latter compound was found to be reduced by NADH in a maleylacetate reductase catalyzed reaction. Maleylacetate and chloride were formed as products of 2-chloromaleylacetate reduction, the former being funnelled into the 3-oxoadipate pathway by a second reductive step. There was no indication for an involvement of a pJP4-encoded enzyme in either the reduction or the dechlorination reaction.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetate

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  1. Institut für Mikrobiologie, Universität Stuttgart, Azenbergstrasse 18, W-7000, Stuttgart 1, Federal Republic of Germany

    Martin Dominik Vollmer, Karin Stadler-Fritzsche & Michael Schlömann

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  1. Martin Dominik Vollmer
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  2. Karin Stadler-Fritzsche
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  3. Michael Schlömann
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Vollmer, M.D., Stadler-Fritzsche, K. & Schlömann, M. Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134. Arch. Microbiol. 159, 182–188 (1993). https://doi.org/10.1007/BF00250280

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