Skip to main content
SpringerLink
Log in

Energetics of glucose uptake in a Salmonella typhimurium mutant containing uncoupled enzyme IIGlc

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP): glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. We have studied the energetics of glucose uptake catalyzed by this uncoupled enzyme IIGlc. The molar growth yields on glucose of two strains cultured anaerobically in glucose-limited chemostat-and batch cultures were compared. Strain PP 799 transported and phosphorylated glucose via an intact PTS, while strain PP 952 took up glucose exclusively via uncoupled enzyme IIGlc, followed by ATP-dependent phosphorylation by glucokinase. Thus the strains were isogenic except for the mode of uptake and phosphorylation of the growth substrate. PP 799 and PP 952 exhibited similar Y Glc values. Assuming equal Y ATP values for both strains this result indicated that there were no energetic demands for glucose uptake via uncoupled enzyme IIGlc.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PTS:

phosphoenolpyruvate: carbohydrate phosphotransferase system

HPr:

histidine-containing phosphocarrier protein

GalP:

galactose permease

References

  • Bauchop T, Elsden SR (1960) The growth of microorganisms in relation to their energy supply. J Gen Microbiol 23: 457–469

    Google Scholar 

  • Bergmeyer HU, Bernt E, Schmidt F, Stock H (1974) d-Glucose. Determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 3. Academic Press, New York, pp 1196–1201

    Google Scholar 

  • Curtis SJ, Epstein W (1975) Phosphorylation of d-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase and glucokinase. J Bacteriol 122: 1189–1199

    Google Scholar 

  • Driessen M, Postma PW, Dam Kvan (1987) Energetics of glucose uptake in Salmonella typhimurium. Arch Microbiol 146: 358–361

    Google Scholar 

  • Erni B (1986) Glucose-specific permease of the bacterial phosphotransferase system: Phosphorylation and oligomeric structure of the glucose specific IIGlc-IIIGlc complex of Salmonella typhimurium. Biochemistry 25: 305–312

    Google Scholar 

  • Gottschalk G (1979) Bacterial fermentations. In: Starr MP (ed) Bacterial metabolism. Berlin Heidelberg New York, pp 167–224

  • Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5B. Academic Press, New York, pp 209–344

    Google Scholar 

  • Peri KG, Kornberg HL, Waygood EB (1984) Evidence for the phosphorylation of enzyme IIGlucose of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium. FEBS Lett 170: 55–58

    Google Scholar 

  • Postma PW (1977) Galactose transport in Salmonella typhimurium. J Bacteriol 129: 630–639

    Google Scholar 

  • Postma PW (1981) Defective enzyme II-BGlc of the phosphoenolpyruvate: sugar phosphotransferase system leading to uncoupling of transport and phosphorylation in Salmonella typhimurium. J Bacteriol 147: 382–389

    Google Scholar 

  • Postma PW, Lengeler JW (1985) Phosphoenolpyruvate: carbohydrate phosphotransferase system of bacteria. Microbiol Rev 49: 232–269

    Google Scholar 

  • Postma PW, Stock JB (1980) Enzymes II of the phosphotransferase system do not catalyze sugar transport in the absence of phosphorylation. J Bacteriol 141: 476–484

    Google Scholar 

  • Postma PW, Schuitema A, Kwa C (1981) Regulation of methyl β-galactoside permease activity in pts and ccr mutants of Salmonella typhimurium. Mol Gen Genet 181: 448–453

    Google Scholar 

  • Tempest DW, Neijssel OM (1984) The status of Y ATP and maintenance energy as biologically interpretable phenomena. Annu Rev Microbiol 38: 459–486

    Google Scholar 

  • Thienen GMvan, Postma PW, Dam Kvan (1979) Proton movements coupled to sugar transport via the galactose transport system in Salmonella typhimurium. Eur J Biochem 73: 521–527

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. E.C. Slater Institute for Biochemical Research, University of Amsterdam, Plantage Muidergracht 12, 1018 TV, Amsterdam, The Netherlands

    G. J. G. Ruijter, P. W. Postma & K. van Dam

Authors
  1. G. J. G. Ruijter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. W. Postma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. van Dam
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ruijter, G.J.G., Postma, P.W. & van Dam, K. Energetics of glucose uptake in a Salmonella typhimurium mutant containing uncoupled enzyme IIGlc . Arch. Microbiol. 155, 234–237 (1991). https://doi.org/10.1007/BF00252206

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00252206

Key words

Search

Navigation