Abstract
Gametes of Chlamydomonas reinhardtii synthesize numerous proteins not observed in vegetative cells and vice versa. Gametogenesis induced changes in gene expression were confirmed by SDS-PAGE of in vitro translation products using total RNA from gametes and vegetative cells. Vegetative cells and gametes thus represent two cell types with distinct patterns of gene expression. The generation of mature gametes from liquid cultures of asynchronously growing vegetative cells was dependent on light. This light requirement could not be substituted for by an organic source of energy and carbon, indicating that light serves as a signal in gametogenesis. The light signal was shown to become effective only after preincubation in nitrogen-free medium. This delayed competence for light indicates that the two external signals — nitrogen-starvation and light —may function in sequence. Execution of the light dependent step in gamete formation required cytoplasmic protein synthesis and RNA synthesis.
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Abbreviations
- CAM:
-
chloramphenicol
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- PSII:
-
photosystem II
- TAP:
-
Tris acetate phosphate
- TMP:
-
Tris minimal phosphate
References
Chua N-H, Gillham NW (1977) The sites of synthesis of the principal thylakoid membrane polypeptides in Chlamydomonas reinhardtii. J Cell Biol 74:441–452
Ferris PJ, Goodenough UW (1987) Transcription of novel genes, including a gene linked to the mating-type locus, induced by Chlamydomonas fertilization. Mol Cell Biol 7:2360–2366
Friedmann I, Colwin AL, Colwin LH (1968) Fine structural aspects of fertilization in Chlamydomonas reinhardtii. J Cell Sci 3:115–128
Gorman DS, Levine RP (1965) Cytochrome and plastocyanin: their sequences in the photosynthetic electron transport chain of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 54:1665–1669
Jones RF (1970) Physiological and biochemical aspects of growth and gametogenesis in Chlamydomonas reinhardtii. Ann New York Acad Sci 175:684–659
Jones RF, Kates JR, Keller SJ (1968) Protein turnover and macromolecular synthesis during growth and gametic differentiation in Chlamydomonas reinhardtii. Biochim Biophys Acta 157:589–598
Kates JR, Jones RF (1964a) Variation in alanine dehydrogenase and glutamate dehydrogenase during the synchronous development of Chlamydomonas. Biochim Biophys Acta 86:438–447
Kates JR, Jones RF (1964b) The control of gametic differentiation in liquid cultures of Chlamydomonas. J Cell Comp Physiol 63:157–164
Kooijman R, Elzenga TJM, de Wildt P, Musgrave A, Schuiring F, van den Ende H (1986) Light dependence of sexual agglutinability in Chlamydomonas eugametos. Planta 169:370–378
Kooijman R, de Wildt P, Homan WL, Musgrave A, van den Ende H (1988) Light affects flagellar agglutinability in Chlamydomonas eugametos by modification of the agglutinin molecules. Plant Physiol 86:216–223
Levine RP, Ebersold WT (1960) The genetics and cytology of Chlamydomonas. Annu Rev Microbiol 14:197–216
Martin NC, Goodenough UW (1975) Gametic differentiation in Chlamydomonas reinhardtii. I. Production of gametes and their fine structure. J Cell Biol 67:587–605
Martin NC, Chiang K-S, Goodenough UW (1976) Turnover of chloroplast and cytoplasmic ribosomes during gametogenesis in Chlamydomonas reinhardtii. Dev Biol 51:190–201
McMahon D (1975) Cycloheximide is not a specific inhibitor of protein synthesis in vivo. Plant Physiol 55:815–821
Picard-Bennoun M, Bennoun P (1985) Change in the cytoplasmic ribosome properties during gametogenesis in the alga Chlamydomonas reinhardtii. Curr Genet 9:239–243
Sager R, Granick S (1954) Nutritional control of sexuality in Chlamydomonas reinhardtii. J Gen Physiol 37:729–742
Schmeisser ET, Baumgartel DM, Howell SH (1973) Gametic differentiation in Chlamydomonas reinhardtii: Cell cycle dependency and rates in attainment of mating competency. Dev Biol 31:31–37
Siersma PW, Chiang K-S (1971) Conservation and degradation of cytoplasmic and chloroplast ribosomes in Chlamydomonas reinhardtii. J Mol Biol 58:167–185
Spreitzer RJ, Mets L (1981) Photosynthesis deficient mutants of Chlamydomonas reinhardtii with associated light-sensitive phenotypes. Plant Physiol 67:565–569
Wegener D, Treier U, Beck CF (1989) Procedures for the generation of mature Chlamydomonas reinhardtii zygotes for molecular and biochemical analysis. Plant Physiol 90:512–515
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This paper is dedicated by C. F. Beck to Professor John L. Ingraham, teacher and friend, on the occasion of his 65th birthday
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Treier, U., Fuchs, S., Weber, M. et al. Gametic differentiation in Chlamydomonas reinhardtii: light dependence and gene expression patterns. Arch. Microbiol. 152, 572–577 (1989). https://doi.org/10.1007/BF00425489
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DOI: https://doi.org/10.1007/BF00425489