Abstract
Thymidylate synthetase mutants of Xanthomonas maltophilia ATCC 13270 were isolated on a solid minimal medium containing 50 mg/l thymidine and a high concentration of trimethoprim (500 mg/l). It was found that a high concentration of trimethoprim was required to prevent background growth of the wild-type strain. The isolated mutants could grow on thymidine or dTMP at a concentration of 50 mg/l while they were unable to grow on 1000 mg/l thymine or 50 mg/l deoxyridine. Thymidylate synthetase activity was assayed in the wild-type cells and in the mutant cells but only the wild-type cells contained measurable enzyme activity.
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West, T.P. Isolation and characterization of thymidylate synthetase mutants of Xanthomonas maltophilia . Arch. Microbiol. 151, 220–222 (1989). https://doi.org/10.1007/BF00413133
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DOI: https://doi.org/10.1007/BF00413133