Abstract
Two ornithine carbamoyltransferases (OCT 1 and OCT 2) were isolated from Pseudomonas syringae pv. phaseolicola and purified by precipitation with ammonium sulfate, heat denaturation, chromatography on DEAE-Sephadex A-50 and Sephadex G-200. Molecular weights of both enzymes: 110,000; optimal activity: pH 8.5 to 9.5 (OCT 1), pH 8.4 (OCT 2); apparent K m for ornithine: 7·10-4 (both enzymes); apparent K m for carbamoylphosphate: 7·10-4 (OCT 1), 2.8·10-3 (OCT 2). Both enzymes possess only an anabolic function. OCT 1 is highly inhibited by low concentrations of phaseolotoxin and Orn-P(O)(NH2)-NH-SO3H, OCT 2 is insensitive to both compounds. The inhibition of OCT 1 is reversible.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PNSOrn:
-
Ornithine-δ-P(O)(NH2)-NH-SO3H
References
Archibald RM (1944) Determination of citrulline and allantoin and demonstration of citrulline in blood plasma. J Biol Chem 156:121–142
Bublitz F, Völksch B (1983) Bildung von Phaseolotoxin and (N-Phosphosulfamyl)ornithin durch Pseudomonas syringae pv. phaseolicola in Submerkultur. Z. Allgem Mikrobiol 23:485–490
Ceriotti G (1970) Ornithin-Carbamyl-Transferase. In: Bergmeyer HU (ed) Methoden der enzymatischen Analyse, vol I. Akademie-Verlag, Berlin, pp 654–658
Conway EI (1957) Microdiffusion analysis and volumetric error. Cooxby and Lockwood, London
Ferguson AR, Johnston JS, Mitchell RE (1980) Resistance of Pseudomonas syringae pv. phaseolicola to its own toxin, phaseolotoxin. FEMS Microbiol Lett 7:123–125
Gnanamanickam SS, Patil SS (1976) Bacterial growth, toxin production and levels of ornithine carbamoyltransferase in resistent and susceptible cultivars of bean inoculated with Pseudomonas phaseolicola. Phytopath 66:290–294
Jahn O (1984) Untersuchungen zur Rolle der Ornithin Carbamoyltransferase (EC 2.1.3.3) von Pseudomonas syringae pv. phaseolicola bei der Resistenz gegen Phaseolotoxin und (N-phosphosulfamyl)ornithin. Dissertation, Univ Jena
Jahn O, Sauerstein J, Reuter G (1985) Detection of two ornithine carbamoyltransferase in a phaseolotoxin producing strain of Pseudomonas syringae pv. phaseolicola. J Basic Microbiol 25:543–546
Kwok OCH, Ako H, Patil SS (1979) Inactivation of bean ornithine carbamoyltransferase by phaseolotoxin: effects of phosphate. Biochem Biophys Res Commun 89:1361–1368
Laplace F, Jahn O, Bublitz F, Sauerstein J, Schluttig A (1985) Die Hemmung der Ornithin-Carbamoyltransferase von Pseudomonas syringae pv. phaseolicola durch das von ihm gebildete N-Phosphosulfamyl-ornithin. Biol Zbl 104:51–56
Legrain C, Stalon V, Noullez JP, Mercenier A, Simon JP, Broman K, Wiame JM (1977) Structure and function of ornithine carbamoyltransferases. Eur J Biochem 80:401–409
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor New York, pp 466
Mitchell RE (1979) Bean halo blight: comparisation of phaseolotoxin and N-phosphoglutamate. Physiol Plant Pathol 14:119–128
Mitchell RE, Bieleski RL (1977) Involvement of phaseolotoxin in halo blight of beans: transport and conversion to functional toxin. Plant Physiol 60:723–729
Mitchell RE, Parsons EA (1977) A naturally occurring analogue of phaseolotoxin (halo blight toxin). Phytochem 16:280–281
Moore RE, Niemczura WP, Kwok OCH, Patil SS (1984) Inhibitors of ornithine carbamoyltransferase from Pseudomonas syringae pv. phaseolicola. Revised structure of phaseolotoxin. Tetrahed Lett 25:3931–3934
Nüske J (1984) Physiologie des Wachstums und der Phaseolotoxin-bildung durch Pseudomonas syringae pv. phaseolicola. Dissertation, Univ Jena
Panopoulos NJ, Staskawicz BJ (1979) Illicit transport of phaseolotoxin in bacterial cells: implications in host-parasite interactions. Proc IVth Int Conf Plant Pathog Bacteriol 2:663–666
Patil SS, Tam LA (1972) Mode of action of the toxin from Pseudomonas phaseolicola. I. Toxin specificity, chlorosis and ornithine accumulation. Plant Physiol 49:803–807
Patil SS, Kolatukudy PE, Dimond AE (1970) Inhibition of ornithine carbamoyltransferase from bean plants by the toxin of Pseudomonas phaseolicola. Plant Physiol 46:752–753
Patil SS, Hayward AC, Emmons R (1974) An ultraviolet induced non-toxingenic mutant of Pseudomonas phaseolicola of altered pathogenicity. Phytophathology 64:590–595
Ramos F, Stalon V, Pierard A, Wiame JM (1967) The specialization of the two ornithine carbamoyltransferase in Pseudomonas. Biochim Biophys Acta 139:98–106
Rudolph K, Stahmann MA (1966) The accumulation of l-ornithine in halo blight-infected bean plants (Phaseolus vulgaris L) induced by the toxin of the pathogen Pseudomonas phaseolicola (Burkh.). Phytophath Z 57:29–46
Sands DO, Schroth MN, Hildebrand DC (1970) Taxonomy of phytopathogenic pseudomonads. J Bacteriol 101:9–23
Staskawicz BJ, Panopoulos NJ, Hoogenraad NJ (1980) Phaseolotoxin-insensitive ornithine carbamoyltransferase of Pseudomonas syringae pv. phaseolicola: basis for immunity to phaseolotoxin. J Bacteriol 142:720–723
Tam LO, Patil SS (1972) Mode of action of the toxin from Pseudomonas phaseolicola. II. Mechanism of inhibition of bean ornithine carbamoyltransferase. Plant Physiol 49:808–812
Young JM, Dye DW, Bradbury JF, Panagopoulos GC, Robbs CF (1978) A proposed nomenclature and classification for plant pathogenic bacteria. NZJ Agric Res 21:153–177
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jahn, O., Sauerstein, J. & Reuter, G. Characterization of two ornithine carbamoyltransferases from Pseudomonas syringae pv. phaseolicola, the producer of phaseolotoxin. Arch. Microbiol. 147, 174–178 (1987). https://doi.org/10.1007/BF00415280
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00415280