Abstract
Production of the macrolide antibiotic tylosin byStreptomyces T59-235 was inhibited in cultures containing high phosphate concentrations (30 mM Pi). Vegetative growth (dry weight increase, DNA and RNA synthesis) was hardly affected. Tylosin production began when macromolecule synthesis had slowed down to minimum level; in cultures with 30 mM Pi the onset of antibiotic production was retarded compared to cultures with low phosphate concentration (5 mM). The activities of three enzyme systems involved in tylosin biosynthesis (dTDP-D-glucose-4,6-dehydratase; dTDP-mycarose-forming enzyme system; SAM: macrocin-O-methyl transferase) were measured and found to be significantly lower in cultures with 30 mM Pi than in low phosphate cultures.
Chloramphenicol, but not rifampicin, caused a rapid decrease of both tylosin formation rate and dTDP-D-glucose-4,6-dehydratase activity when added to tylosin producing cultures.
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Abbreviations
- Pi :
-
inorganic phosphate
- dTDB:
-
2′-deoxythymidine diphosphate
- SAM:
-
S-adenosyl-L-methionine
- LP:
-
low phosphate (5 mM)
- HP:
-
high phosphate (30 mM)
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Madry, N., Pape, H. Formation of secondary metabolism enzymes in the tylosin producerStreptomyces T59-235. Arch. Microbiol. 131, 170–173 (1982). https://doi.org/10.1007/BF01054001
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DOI: https://doi.org/10.1007/BF01054001