Abstract
The effect of the tryptophan analogue 4-methyltryptophan in Saccharomyces cerevisiae has been investigated. 4-Methyltryptophan inhibits the aminoacylation of tryptophan specific transfer ribonucleic acid (tRNATrp). The mode of inhibition is competitive and the analogue is not charged onto tRNATrp. Thus 4-methyltryptophan application depletes the cells from charged tRNATrp. As a consequence cell growth and protein synthesis are strongly reduced. 4-Methyltryptophan is degraded efficiently in culture media inoculated with the wild type strain; the effects of 4-methyltryptophan were therefore found to be transient.
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References
Ehresmann B, Imbault P, Weil JH (1974) Determination of the in vivo tRNA aminoacylation in yeast cells. Anal Biochem 61:548–556
Fantes P, Roberts LM, Hütter R (1976) Free tryptophan pool and tryptophan biosynthetic enzymes in Saccharomyces cerevisiae. Arch Microbiol 107:207–214
Greasham RL, Moat AG (1973) Amino acid transport in a polyaromatic amino acid auxotroph of Saccharomyces cerevisiae. J Bacteriol 115:975–981
Grenson M, Hou C, Crabeel M (1970) Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease. J Bacteriol 103:770–777
Hagemann F (1969) Vergleichende Untersuchungen zur Umsetzung von d- und l-Tryptophan sowie von N-Acetyl-Tryptophan an ausgewählten Hefearten. Inaugural-Dissertation der Justus Liebig-Univ., Gießen
Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, Vol. 5 B. Academic Press, New York-London, pp 242–265
Keith G, Gargloff J, Dirheimer G (1971) Conditions optimales d'aminoacylation du tRNAAsp et du tRNATrp de levure. Biochimie 53:661–669
Miozzari G, Niederberger P, Hütter R (1977) Action of tryptophan analogues in Saccharomyces cerevisiae. Arch Microbiol 115:307–316
Nass G, Poralla K (1976) Genetics of borrelidin resistant mutants of Saccharomyces cerevisiae and properties of their threonyl-tRNA-synthetase. Molec Gen Genet 147:39–43
Shetty AS, Gaertner FH (1973) Distinct kynureninase and hydroxykynureninase activities in microorganisms: Occurrence and properties of a single physiologically discrete enzyme in yeast. J Bacteriol 113:1127–1133
Schürch AR (1972) Zur Regulation der Tryptophan-Biosynthese bei Saccharomyces cerevisiae. Diss. ETHZ 4862. Juris Druck und Verlag. Zürich
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Stäheli, P., Kradolfer, P., Niederberger, P. et al. Inhibition of yeast tRNATrp aminoacylation by 4-methyltryptophan. Arch. Microbiol. 129, 146–149 (1981). https://doi.org/10.1007/BF00455351
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DOI: https://doi.org/10.1007/BF00455351