Abstract
Beneckea natriegens oxidizes sodium formate constitutively when grown on glucose or glycerol in chemostat culture, but cannot utilize formate as the sole source of carbon and energy for growth. However, when grown on a mixture of glucose and formate (D=0.37 h-1, pH 7.6) the yield is higher than on glucose alone.
The yield, expressed in terms of g bacterial dry weight g-1 glucose plus formate carbon utilized, gave a linear relationship when plotted against the total heat of combustion of glucose plus formate utilized. Extrapolation of the plot cut the abscissa at a value equivalent to the heat of combustion of formate, which suggests that formate is not utilised as a source of carbon but only energy.
In cultures with nitrate as the sole source of nitrogen the yield from glucose was lower than that observed with ammonia but the addition of formate to the culture utilizing nitrate resulted in an increase in the yield from glucose to a value similar to that observed with ammonia.
At a culture pH value of 7.65 unused formate (<0.15–227 mM) in the culture supernatant had no effect on respiration spiration or yield, but at a culture pH of 6.7 excess formate caused a marked increase in respiration rate and a large decrease in the yield from glucose; further decrease in the pH value caused washout of the culture. This may be explained by undissociated formic acid causing uncoupling of oxidative phosphorylation.
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References
Harder W, Wiersma M, Groen L (1974) Transport of substrates and energetics of growth of Pseudomonas oxalaticus during growth on formate or oxalate in continuous culture. J Gen Microbiol 81:ii
Harrison DEF (1976) The regulation of respiration rate in growing bacteria. In: AH Rose, DW Tempest (eds) Advances in Microbial Physiology, Vol 14, Academic Press New York, pp 243–313
Hueting S, Tempest DW (1977) Influence of acetate on the growth of Candida utilis in continuous culture. Arch Microbiol 115:73–78
Jones CW, Brice JM, Edwards C (1977) The effect of respiratory chain composition on the growth efficiency of aerobic bacteria. Arch Microbiol 115:85–93
Lang E, Lang H (1972) Spezifische Farbreaktion zum direkten Nachweis der Ameisensäure. Z Anal Chem 260:8–10
Law JH, Slepecky RA (1961) Assay of poly-β-hydroxybutyric acid. J Bacteriol 82:33–42
Linton JD, Harrison DEF, Bull AT (1975) Molar growth yields, respiration and cytochrome patterns of Beneckea natriegens when grown at different medium dissolved oxygen tensions. J Gen Microbiol 90:237–246
Linton JD, Harrison DEF, Bull AT (1976) The effect of rate of respiration on sensitivity to cyanide and carbon monoxide in Beneckea natriegens grown in batch and continuous culture. FEBS Lett 64:358–363
Linton JD, Harrison DEF, Bull AT (1977) Molar growth yields, respiration and cytochrome profiles of Beneckea natriegens when grown under carbon limitation in a chemostat. Arch Microbiol 115:135–142
Linton JD, Stephenson RJ (1978) A preliminary study on growth yields in relation to the carbon and energy content of various organic growth substrates. FEMS Lett 3:95–98
Payne WJ, Eagon RG, Williams AK (1961) Some observations on the physiology of Pseudomonas natriegens nov. spec. Antonie van Leeuwenhoek, J Microbiol Serol 27:121–128
Tempest DW (1978) The biochemical significance of microbial growth yields: a reassessment. TIBS 3:180–184
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Linton, J.D., Griffiths, K. & Gregory, M. The effect of mixtures of glucose and formate on the yield and respiration of a chemostat culture of Beneckea natriegens . Arch. Microbiol. 129, 119–122 (1981). https://doi.org/10.1007/BF00455345
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DOI: https://doi.org/10.1007/BF00455345