Abstract
Acetoin dehydrogenase can be catabolite repressed by numerous sources of carbon. The following results point out that the catabolite repression of this enzyme and the inhibition of sporulation are mediated by the same mechanism:
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1.
Mutants, able to synthesize acetoin dehydrogenase in the presence of glucose, sporulate in glucose medium at a higher rate than the standard strain.
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2.
The catabolite repressing effect of a compound and its ability to inhibit sporulation are in a direct relation to each other.
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3.
The limitation of inorganic phosphate in the growth medium, which is known to favour sporulation, counteracts the catabolite repressing effect of glucose.
Zusammenfassung
Das Enzym Acetoin-Dehydrogenase wird von zahlreichen Kohlenstoffquellen katabolisch reprimiert. Die folgenden Bufunde weisen darauf hin, daß die Repression dieses Enzyms und die Inhibition der Sporulation durch den gleichen Mechanismus erfolgen.
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1.
Mutanten, isoliert nach ihrer Fähigkeit, Acetoin Dehydrogense trotz Anwesenheit von Glucose zu bilden, können auch in Glucose Medium in einer höheren Rate als der Wildtyp sporulieren.
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2.
Die Fähigkeit einer Kohlenstoffquelle, die Induktion der Acetoin-Dehydrogenase zu hemmen, ist ihrer sporulationsinhibierenden Wirkung proportional.
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3.
Phosphatlimitierung im Medium, was bekanntlich Sporulation fördert, hat eine der katabolischen Repression entgegengesetzte Wirkung.
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Lopez, J.M., Thoms, B. Beziehungen zwischen katabolischer Repression und Sporulation bei Bacillus subtilis . Arch. Microbiol. 109, 181–186 (1976). https://doi.org/10.1007/BF00425133
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DOI: https://doi.org/10.1007/BF00425133