Abstract
Escherichia coli B/r/l was synchronized by a novel method and its growth was followed in a minimal salts medium containing glucose, acetate, aspartate or succinate as the sole carbon source. Thymine incorporation experiments showed agreement with the Cooper-Helmstetter model for DNA synthesis, during the division cycle, both in glucose grown culture with a doubling time 57.5 min and in acetate, aspartate and succinate where the doubling time was extended up to 90 min. The ratio C/C+D was identical or close to that predicted by the model. Prolonged growth of the synchronized cultures prior to each experiment was practised in order to ensure their physiological state without causing any considerable deterioration of synchrony.
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References
Anagnostopoulos, G. D.: Unbalanced growth in a semi-continuous culture system designed for the synchronization of cell division. J. gen. Microbiol. 65, 23–33 (1971)
Anagnostopoulos, G. D.: Defining synchrony of cell cultures. Arch. Microbiol. 104, 95–96 (1975)
Anagnostopoulos, G. D.: Induction and stabilization of synchrony in the cell division of Escherichia coli. Arch. Microbiol. 107, 199–203 (1976)
Buckley, D. E., Anagnostopoulos, G. D.: Growth of Escherichia coli B/r/l in a semi-continuous system designed for the synchronization of cell division. Arch. Microbiol. 105, 169–172 (1975)
Chai, N. S., Lark, K. G.: Cytological studies of deoxynucleic acid replication in Escherichia coli 15T-: Replication at slow growth rates and after a shift-up into rich medium. J. Bact. 104, 401–409 (1970)
Clark, D. J.: The regulation of DNA replication and cell division in E. coli B/r. Cold Spr. Harb. Symp. quant. Biol. 33, 823–836 (1968)
Cooper, S., Helmstetter, C. E.: Chromosome replication and the division cycle of Escherichia coli B/r. J. molec. Biol. 31, 519–540 (1968)
Gudas, L. J., Pardee, A. B.: Deoxyribonucleic acid synthesis during the division cycle Escherichia coli: A comparison of strains B/r, K-12, 15 and 15T- under conditions of slow growth. J. Bact. 117, 1216–1223 (1974)
Helmstetter, C. E., Cooper, S.: DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J. molec. Biol. 31, 507–518 (1968)
Helmstetter, C. E., Cooper, S., Pierucci, O., Revelas, E.: On the bacterial life sequence. Cold Spr. Harb. Symp. quant. Biol. 33, 809–822 (1968)
Jones, N. C., Donachie, W. D.: Chromosome replication, transcription and control of cell division. Nature New Biol. 243, 100–103 (1973)
Kubitschek, H. E., Bendigkeit, H., Loken, M.: Onset of DNA synthesis during the cell cycle in chemostal cultures. Proc. nat. Acad. Sci. (Wash.) 57, 1611–1617 (1967)
Lark, C.: Regulation of deoxyribonucleic acid in Escherichia coli: Dependence on growth rates. Biochim. biophys. Acta (Amst.) 119, 517–525 (1966)
Pierucci, O.: Chromosome replication and cell division in Escherichia coli at various temperatures of growth. J. Bact. 109, 848–854 (1972)
Pirt, S. J.: A kinetic study of the mode of growth of surface colonies of bacteria and fungi. J. gen. Microbiol. 47, 181–197 (1967)
Scherbaum, O. H.: Comparison of synchronous and synchronized cell division. Exp. Cell Res. 33, 89–98 (1964)
Smith, H. S., Pardee, A. B.: Accumulation of a protein required for division during the cell cycle of Escherichia coli. J. Bact. 101, 901–909 (1970)
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Buckley, D.E., Anagnostopoulos, G.D. DNA synthesis by Escherichia coli B/r/l synchronized and grown under conditions of slow growth. Arch. Microbiol. 109, 143–146 (1976). https://doi.org/10.1007/BF00425126
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DOI: https://doi.org/10.1007/BF00425126