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Genetics and mapping of new isozyme loci in Vicia faba L using trisomics

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Abstract

Polymorphism in ten enzyme systems (ACO, ACP, AAT, EST, FK, ME, NAG, PRX, 6PGD, and SOD) in Vicia faba L. was analyzed, revealing 13 loci, six of which have not been reported before. Inheritance, genetics, possible location, and linkage analysis were studied in 13 different F2 populations trisomic for four of the six chromosomes (nos. 3, 4, 5, and 6) of the species. Each of these loci exhibited typical Mendelian inheritance except for those involved in the trisomic chromosome. Five loci have been assigned to a specific chromosome: Est-2 to chromosome 3, Fk-2 to chromosome 4, Prx-1 to chromosome 5, and Sod-1 and Pgd-p to chromosome 6. Nag-1 and Pgd-c displayed a linkage of 22.8 cM indicating a clear homology with chromosome 5 of garden pea on which both markers are syntenic.

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References

  • Bassiri A, Rouhani I (1977) Identification of broad-bean cultivars based on isoenzyme patterns. Euphytica 26:279–286

    Google Scholar 

  • Cabrera A, Martín A (1989) Analysis of genetic linkage in faba bean (Vicia faba L.). FABIS Newslett 24:3–5

    Google Scholar 

  • Cabrera A, Cubero JI, Martín A (1989) Genetic mapping using trisomics in Vicia faba L. FABIS Newslett 23:5–7

    Google Scholar 

  • Cardy BJ, Stuber CW, Goodman MM (1980) Techniques for starchgel electrophoresis of enzymes from maize (Zea mays L.). Department of statistics, Mimeo Series, North Carolina State University, Raleigh

    Google Scholar 

  • Carre S, Tasei JN, Mesquida J, Le Guen J (1991) Estimate of outcrossing rate between two lines of field beans (Vicia faba) in various conditions with isozymic markers. In: 6th Int Symp Pollination, 27–31 August 1990. Research Centre for Insect Pollination and Beekeeping and International Society for Horticultural Science, Tilburg, Netherlands, pp 354–358

    Google Scholar 

  • Clayton JW, Tretiak DN (1972) Amino-citrate buffers for pH control in starch-gel electrophoresis. J Fish Res Board Can 29: 1169–1172

    CAS  Google Scholar 

  • Gates P, Boulter D (1979) The use of seed isoenzymes as an aid to the breeding of field beans (Vicia faba L.). New Phytol 83: 783–791

    Google Scholar 

  • Gates P, Boulter D (1980) The use of pollen isoenzymes as an aid to the breeding of field beans (Vicia faba L.). New Phytol 84: 501–504

    Google Scholar 

  • Gottlieb LD (1973) Enzyme differentiation and phylogeny in Clarkia franciscana, C. rubicunda and C. amoena. Evolution 27: 205–214

    Google Scholar 

  • Hermsen JB (1970) Basic information for the use of primary trisomics in genetics and breeding research. Euphytica 19:125–140

    Google Scholar 

  • International Union of Biochemistry Nomenclature Committee (IUB) (1984) Enzyme Nomenclature. Academic Press, New York

    Google Scholar 

  • Kaser HR, Steiner AM (1983) Subspecific classification of Vicia faba L. by protein and isozyme patterns. FABIS Newslett 7:19–20

    Google Scholar 

  • Ladizinsky G (1975) Seed-protein electrophoresis of the wild and cultivated species of section Faba of Vicia. Euphytica 24: 785–788

    Google Scholar 

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage groups. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER; an interactive computer program for constructing genetic linkage maps of experimental and natural populations. Genomics 1:174

    CAS  PubMed  Google Scholar 

  • Mancini R, De Pace C, Scarascia GT, Delre V, Vitori D (1989) Isozyme gene markers in Vicia faba L. Theor Appl Genet 77:657–667

    Google Scholar 

  • Martín A, Barceló P (1984) The cytology and morphology of Vicia faba trisomics. In: Chapman GP, Tarawai SA (eds) Systems for cytogenetic analysis in Vicia faba L. Martinus Nijhoff, The Hague, pp 63–76

    Google Scholar 

  • Mather K (1957). The measurement of linkage in heredity, 3rd edn. Methuen, London

    Google Scholar 

  • Morton NE (1956) Sequential tests for the detection of linkage. Am J Hum Genet 7:277–318

    Google Scholar 

  • Muehlbauer FJ, Weeden NF, Hoffman DL (1989) Inheritance and linkage relationships of morphological and isozyme loci in lentil (Lens Miller). J Hered 80:298–303

    Google Scholar 

  • Peat WE, Adham IY (1984) The use of isozyme genes as markers in the population genetics of Vicia faba L. In: Chapman GP, Tarawai SA (eds) Systems for cytogenetic analysis in Vicia faba L. Advances in Agricultural Biotechnology vol II. M. Nijhoff, Dordrecht, The Netherlands, pp 109–117

    Google Scholar 

  • Polignano GB, Sonnante G (1992). Characterization of faba-bean populations by isoenzyme patterns of GOT and PGI. FABIS Newslett 31:12–16

    Google Scholar 

  • Selander RK, Smith MH, Yang SY, Johnson WE, Gentry JB (1971) Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-field mouse (Peromyscus polionotus). Univ Texas Publ 7103:49–90

    Google Scholar 

  • Sjödin J (1971) Induced translocation in Vicia faba L. Herditas 68:1–34

    Google Scholar 

  • Suiter K, Wendel J, Case J (1983) LINKAGE-1: a Pascal computer program for the detection and analysis of genetic linkage. J Hered 74:203–204

    Google Scholar 

  • Suso MJ, Moreno MT (1982) Genetic control of electrophoretic variants of glutamate oxalacetate transaminase (GOT) in Vicia faba L. FABIS Newslett 5:14

    Google Scholar 

  • Suso MJ, Moreno MT (1995) Outcrossing rate in two faba-bean cultivars under dryland conditions in Spain. Can J Plant Sci 75 (inpress)

  • Suso MJ, Moreno MT (1986). Isoenzymatic polymorphism of superoxidase dismutase (SOD) in Vicia faba and its systematic implication. FABIS Newslett 16:3–5

    Google Scholar 

  • Suso MJ, Moreno MT, Cubero JI (1993) New isozyme markers in Vicia faba: inheritance and linkage. Plant Breed 111:170–172

    Google Scholar 

  • Torres AM, Weeden NF, Martín A (1993a) Linkage among Isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85:937–945

    Google Scholar 

  • Torres AM, Millán T, Cabrera A, Cubero JI (1993b) Establecimiento de nuevos grupos de ligamiento en Vicia faba. XXVIII. Jornadas Luso-Espanholas de Genética, Universidade do Algarve, Faro, Portugal, 169

    Google Scholar 

  • Van deVen WTG, Waugh R, Duncan N, Ramsay G, Dow N, Powel W (1991) Development of a genetic linkage map in Vicia faba using molecular and biochemical techniques. Asp Appl Biol 27:49–54

    Google Scholar 

  • Weeden NF (1988) A suggestion for the nomenclature of isozyme loci. Pisum Newslett 20:44–45

    Google Scholar 

  • Weeden NF, Gottlieb LD (1980) Isolation of cytoplasmic enzymes from pollen. Plant Physiol 66:400–403

    Google Scholar 

  • Weeden NF, Marx GA (1987) Further genetic analysis and linkage relationships of isozyme loci in the pea. J Hered 78:153–159

    Google Scholar 

  • Weeden NF, Wolko B (1990) Linkage map for the garden pea (Pisum sativum) based on molecular markers. In: O'Brien SJ (ed) Locus maps of complex genomes, genetic maps, 5th edn., book 6, Plants. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 106–112

    Google Scholar 

  • Wendel JF, Weeden NF (1990) Visualization and interpretation of plant isozymes. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Dioscorides Press, Portland, Oregon, pp 5–45

    Google Scholar 

  • Yamamoto K (1975) Estimation of genetic homogeneity by isozymes from interspecific hybrids of Vicia. I. Amylase isozyme patterns in the hybrid progenies between Vicia pilosa and Vicia macrocarpa. Jpn J Breed 25:60–64

    Google Scholar 

  • Yamamoto K (1979) Estimation of genetic homogeneity by isozymes from interspecific hybrids of Vicia. II. Amylase isozyme patterns in the hybrid progenies between Vicia amphicarpa and V. macrocarpa. Jpn J Breed 29:59–65

    Google Scholar 

  • Yamamoto K, Plitmann U (1980) Isozyme polymorphism in species of the genus Vicia (Leguminosae). Jpn J Genetics 55:151–164

    Google Scholar 

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Communicated by J. W. Snape

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Torres, A.M., Satovic, Z., Canovas, J. et al. Genetics and mapping of new isozyme loci in Vicia faba L using trisomics. Theoret. Appl. Genetics 91, 783–789 (1995). https://doi.org/10.1007/BF00220960

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